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. 2018 Mar 2;80(2):375-381.
doi: 10.1292/jvms.17-0410. Epub 2017 Dec 27.

Sex and site differences in urinary excretion of conjugated pyrene metabolites in the West African Shorthorn cattle

Nesta Bortey-Sam  1 , Yoshinori Ikenaka  1   2 , Osei Akoto  3 , Shouta M M Nakayama  1 , Jemima T Marfo  1 , Aksorn Saengtienchai  4 , Hazuki Mizukawa  1 , Mayumi Ishizuka  1
Affiliations

Sex and site differences in urinary excretion of conjugated pyrene metabolites in the West African Shorthorn cattle

Nesta Bortey-Sam et al. J Vet Med Sci. .

Abstract

Industrialization, economic and population growth rates in Ghana have increased the release of contaminants including polycyclic aromatic hydrocarbons (PAHs) into the environment through which humans and animals are exposed. Cattle is reported to be exposed to high levels of PAHs through feed and inhalation. Once exposed, PAHs are metabolized and excreted in urine, feces or bile. In a previous study, cattle in Ghana was reported to excrete high levels of 1-hydroxypyrene (1-OHPyr) due to high exposure to the parent compound, pyrene. 1-OHPyr is further metabolized to glucuronide and sulfate conjugates. Thus, the aim of this study was to investigate the sex and site differences in urinary excretion of conjugated pyrene metabolites using cattle urine collected from rural and urban sites of the Ashanti region, Ghana. From the results, geometric mean concentration adjusted by specific gravity indicated that 1-OHPyreneGlucuronide (PyG) was the most abundant conjugate followed by PyrenediolSulfate (M3). The sum of conjugated pyrene metabolites and sum of both conjugated and deconjugated pyrene metabolites correlated significantly with PyG, PydiolSulfate (M2) and PydiolSulfate (M3). The study revealed no significant difference in urinary excretion of conjugated pyrene metabolites between rural and urban sites. This indicated that similar to urban sites, cattle in rural sites were exposed to high levels of pyrene. There was no significant difference in urinary concentrations of conjugated pyrene metabolites between sexes.

Keywords: Kumasi; PAHs; cattle; metabolites; urine.

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Figures

Fig. 1.
Fig. 1.
Map showing cattle urine sampling locations in the Ashanti Region, Ghana (yellow pins indicate sampled locations and red pin indicates city center in Kumasi) (Obtained from Bortey-Sam et al. [6]).
See this image and copyright information in PMC

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