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. 2017 May-Jun;20(3):152-156.
doi: 10.4103/0972-0707.218303.

Cytocompatibility of a self-adhesive gutta-percha root-filling material

Joyce Nascimento  1 , Miriam Zaccaro Scelza  1 , Gutemberg Gomes Alves  1 , Adriana Linhares  1 , Antonio Canabarro  2 , Jose Mauro Granjeiro  3 , Gustavo De-Deus  4
Affiliations

Cytocompatibility of a self-adhesive gutta-percha root-filling material

Joyce Nascimento et al. J Conserv Dent. 2017 May-Jun.

Abstract

Context: A novel root-filling material based on the incorporation of ultrafine alkaline bioactive glass particles (bioactive gutta-percha, [BGP]) was developed to work without sealer.

Aim: In the present study, the objective was to verify the in vitro biological response to this material by assessing its cytocompatibility.

Materials and methods: Prototypes of BGP were compared to conventional gutta-percha (GP), dense polystyrene beads as a negative control and fragments of latex as a positive control. Extracts of each material were prepared according to ISO 10993-5:2009, and human osteoblast-like cells in primary culture were exposed to all extracts for 24 h. Cell viability was assayed sequentially for three different parameters: mitochondrial activity, membrane integrity, and cell density.

Statistical analysis used: Nonparametric analysis (using Kruskal-Wallis test combined with post hoc Dunn's test) was performed for comparison among groups, with significance established at 5%.

Results: BGP reduced mitochondrial activity to 62% of control, but presented no toxicity on membrane integrity and proliferation assays. BGP effect on metabolism was dose-dependent and reduced to acceptable levels with dilution.

Conclusion: The novel GP material presented slight dose-dependent effects on cell metabolism but did not affect cell survival.

Keywords: Biocompatibility testing; bioglass; gutta-percha.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Multiparametric cytotoxicity assay. (a) XTT; (b) NR and (c) CVDE. Bars represent mean ± standard deviation (n = 5), normalized as a percentage of the control (untreated) group. PS – Polystyrene: Negative control; Latex: Positive control; BGP: Bioglass gutta-percha; GP: Gutta-percha. An asterisk indicates significant difference from control group(P < 0.05)
Figure 2
Figure 2
Determination of dependence of dose on the XTT assay. Extracts were diluted on culture media in the indicated percentage before cell exposure. Results of XTT reduction presented as mean ± standard deviation (n = 5), normalized as a percentage of the control (untreated) group. Latex: Positive control; BGP: Bioglass gutta-percha; GP: Gutta-percha
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