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. 2017 Dec 27;12(12):e0190106.
doi: 10.1371/journal.pone.0190106. eCollection 2017.

CD14-159C/T polymorphism in the development of delayed skin hypersensitivity to tuberculin

Magdalena Druszczynska  1 , Marcin Wlodarczyk  1 , Grzegorz Kielnierowski  2 , Michal Seweryn  3 , Sebastian Wawrocki  1 , Wieslawa Rudnicka  1
Affiliations

CD14-159C/T polymorphism in the development of delayed skin hypersensitivity to tuberculin

Magdalena Druszczynska et al. PLoS One. .

Abstract

The skin tuberculin test (TST), an example of a delayed-type hypersensitivity (DTH) reaction, is based on measuring the extent of skin induration to mycobacterial tuberculin (PPD). Little is known about the genetic basis of TST reactivity, widely used for diagnosing TB infection. The study investigated the relationship of the single base change polymorphic variants in CD14 gene (CD14(-159C/T)) with the development of DTH to PPD in BCG-vaccinated Polish Caucasian individuals. We found persistent lack of TST reactivity in about 40% of healthy subjects despite receiving more than one dose of BCG. The TST size was negatively correlated with the number of BCG inoculations. The distribution of C/T genotype was significantly more frequent among TST-negative compared with TST-positive individuals. The concentration of serum sCD14 was positively associated with mCD14 expression, but not with the TST status or CD14(-159C/T) polymorphism. A significant increase in mCD14 expression and serum sCD14 levels was found in TB group. We hypothesize that CD14(-159C/T) polymorphic variants might be one of genetic components in the response to attenuated M. bovis BCG bacilli.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Correlation between TST size and the number of BCG doses in the group of healthy, young individuals.
Fig 2
Fig 2. Distribution of TST sizes among the study groups: TB patients (black bars), non-TB patients (grey bars) and young healthy volunteers (white bars).
Fig 3
Fig 3. Correlation between serum sCD14 levels and mCD14 expression in individuals with C/C, C/T and T/T CD14(-159C/T) genotypes.
See this image and copyright information in PMC

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