Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Apr;86(7):2301-5.
doi: 10.1073/pnas.86.7.2301.

Polycythemia in transgenic mice expressing the human erythropoietin gene

G L Semenza  1 , M D Traystman, J D Gearhart, S E Antonarakis
Affiliations

Polycythemia in transgenic mice expressing the human erythropoietin gene

G L Semenza et al. Proc Natl Acad Sci U S A. 1989 Apr.

Abstract

Erythropoietin is a glycoprotein hormone that regulates mammalian erythropoiesis. To study the expression of the human erythropoietin gene, EPO, 4 kilobases of DNA encompassing the gene with 0.4 kilobase of 5' flanking sequence and 0.7 kilobase of 3' flanking sequence was microinjected into fertilized mouse eggs. Transgenic mice were generated that are polycythemic, with increased erythrocytic indices in peripheral blood, increased numbers of erythroid precursors in hematopoietic tissue, and increased serum erythropoietin levels. Transgenic homozygotes show a greater degree of polycythemia than do heterozygotes as well as striking extramedullary erythropoiesis. Human erythropoietin RNA was found not only in fetal liver, adult liver, and kidney but also in all other transgenic tissues analyzed. Anemia induced increased human erythropoietin RNA levels in liver but not kidney. These transgenic mice represent a unique model of polycythemia due to increased erythropoietin levels.

PubMed Disclaimer

References

    1. Anal Biochem. 1983 Jul 1;132(1):6-13 - PubMed
    1. J Mol Biol. 1983 Feb 5;163(4):673-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4438-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4 - PubMed
    1. Rev Physiol Biochem Pharmacol. 1986;104:139-215 - PubMed

Publication types