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. 2017 Nov 30:6:147.
doi: 10.4103/2277-9175.219412. eCollection 2017.

Pseudomonas aeruginosa-producing Metallo-β-lactamases (VIM, IMP, SME, and AIM) in the Clinical Isolates of Intensive Care Units, a University Hospital in Isfahan, Iran

Farzin Khorvash  1 , Mohammadreza Yazdani  1 , Shiva Shabani  2 , Aliasghar Soudi  2
Affiliations

Pseudomonas aeruginosa-producing Metallo-β-lactamases (VIM, IMP, SME, and AIM) in the Clinical Isolates of Intensive Care Units, a University Hospital in Isfahan, Iran

Farzin Khorvash et al. Adv Biomed Res. .

Abstract

Background: Pseudomonas aeruginosa is a severe challenge for antimicrobial therapy, due to the chromosomal mutations or exhibition of intrinsic resistance to various antimicrobial agents such as most β-lactams. We undertook this study to evaluate the existence of SME, IMP, AIM, and VIM metallo-β-lactamases (MBL) encoding genes among P. aeruginosa strains isolated from Intensive Care Unit (ICU) patients in Al-Zahra Hospital in Isfahan, Iran.

Materials and methods: In a retrospective cross-sectional study that was conducted between March 2012 and April 2013, a total of 48 strains of P. aeruginosa were collected from clinical specimens of bedridden patients in ICU wards. Susceptibility test was performed by disc diffusion method. All of the meropenem-resistant strains were subjected to modified Hodge test for detection of carbapenemases. Multiplex polymerase chain reaction was performed for detection of blaVIM, blaIMP, blaAIM, and blaSME genes.

Results: In disk diffusion method, imipenem and meropenem showed the most and colistin the least resistant antimicrobial agents against P. aeruginosa strains. Of the 48 isolates, 36 (75%) were multidrug resistant (MDR). Amplification of β-lactamase genes showed the presence of blaVIM genes in 7 (%14.6) strains and blaIMP genes in 15 (31.3%) strains. All of the isolates were negative for blaSME and blaAIM genes. We could not find any statistically significant difference among the presence of this gene and MDR positive, age, or source of the specimen.

Conclusion: As patients with infections caused by MBL-producing bacteria are at an intensified risk of treatment failure, fast determination of these organisms is necessary. Our findings may provide useful insights in replace of the appropriate antibiotics and may also prevent MBLs mediated resistance problem.

Keywords: Antimicrobial resistance; Pseudomonas aeruginosa; metallo-β-lactamases.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Antimicrobial resistance rates among Pseudomonas aeruginosa isolates
Figure 2
Figure 2
Gel electrophoresis of multiplex polymerase chain reaction products following amplification with specific primers for blaVIM gene (391bp). Lanes: (2) the 50bp ladder; (3-5) clinical isolates for blaVIM gene, (6) blaVIM gene blaIMP (232bp) positive control, (7) negative control
Figure 3
Figure 3
Gel electrophoresis of multiplex polymerase chain reaction products following amplification with specific primers for blaIMP gene (232bp). Lanes (1) blaIMP (232bp) positive control, (2) the 50bp ladder, (3-6) clinical isolates for blaIMP gene, (7) negative control
Figure 4
Figure 4
Gel electrophoresis of multiplex polymerase chain reaction products following amplification with specific primers for blaVIM gene (391bp). Lanes: (1) the 50bp ladder, (2-5) clinical isolates for blaVIM gene, (6) blaVIM gene blaIMP (232bp) positive control, (7) negative control
See this image and copyright information in PMC

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