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Review
. 2018 Feb;15(2):105-112.
doi: 10.1080/14789450.2018.1421947. Epub 2018 Jan 9.

Proteomic analysis of the cardiac extracellular matrix: clinical research applications

Merry L Lindsey  1   2 , Mira Jung  2 , Michael E Hall  2   3 , Kristine Y DeLeon-Pennell  1   2
Affiliations
Review

Proteomic analysis of the cardiac extracellular matrix: clinical research applications

Merry L Lindsey et al. Expert Rev Proteomics. 2018 Feb.

Abstract

The cardiac extracellular matrix (ECM) provides anatomical, biochemical, and physiological support to the left ventricle. ECM proteins are difficult to detect using unbiased proteomic approaches due to solubility issues and a relatively low abundance compared to cytoplasmic and mitochondrial proteins present in highly prevalent cardiomyocytes. Areas covered: Proteomic capabilities have dramatically improved over the past 20 years, due to enhanced sample preparation protocols and increased capabilities in mass spectrometry (MS), database searching, and bioinformatics analysis. This review summarizes technological advancements made in proteomic applications that make ECM proteomics highly feasible. Expert commentary: Proteomic analysis of the ECM provides an important contribution to our understanding of the molecular and cellular processes associated with cardiovascular disease. Using results generated from proteomics approaches in basic science applications and integrating proteomics templates into clinical research protocols will aid in efforts to personalize medicine.

Keywords: Extracellular matrix; cardiac remodeling; cardiovascular disease; proteomics.

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Conflict of interest statement

Declaration of interest

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript.

References

    1. Spinale FG, Frangogiannis NG, Hinz B, et al. Crossing into the next frontier of cardiac extracellular matrix research. Circ Res. 2016;119:1040–1045. Defines areas of research needed for cardiac extracellular matrix. - PMC - PubMed
    1. Lindsey ML, Hall ME, Harmancey R, et al. Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction. Clin Proteomics. 2016;13:19. Summarizes efforts taken in the proteomics research field that have expanded our understanding of post-MI LV remodeling. - PMC - PubMed
    1. De Castro Bras LE, Ramirez TA, DeLeon-Pennell KY, et al. Texas 3-step decellularization protocol: looking at the cardiac extracellular matrix. J Proteomics. 2013;86:43–52. Defined a decellularizaiton and resolubilization protocol for examining cardiac ECM. - PMC - PubMed
    1. Lindsey ML, Mayr M, Gomes AV, et al. Transformative impact of proteomics on cardiovascular health and disease: a scientific statement from the American Heart Association. Circulation. 2015;132:852–872. Defined the current state of cardiovascular proteomics. - PubMed
    1. Francis Stuart SD, De Jesus NM, Lindsey ML, et al. The crossroads of inflammation, fibrosis, and arrhythmia following myocardial infarction. J Mol Cell Cardiol. 2016;91:114–122. - PMC - PubMed

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