Fluorescence resonance energy transfer aptasensor between nanoceria and graphene quantum dots for the determination of ochratoxin A

Abstract

In the present work, colloidal cerium oxide nanoparticles (nanoceria) and graphene quantum dots (GQDs) were firstly synthesized by sol-gel method and pyrolysis respectively, which all have a uniform nano-size and significant fluorescence emission. Due to the fluorescence emission spectrum of nanoceria overlapped the absorption spectrum of GQDs, fluorescence resonance energy transfer (FRET) between nanoceria and GQDs could occur effectively by the electrostatic interaction. Based on it, a sensitive ratiometric fluorescence aptasensor for the determination of ochratoxin A (OTA), a small molecular mycotoxin produced by Aspergillus and Penicillium strains, has been successfully constructed. In which, probe DNA1@nanoceria and DNA2@GQD were designed to complementary with OTA aptamer, both could adsorb each other, leading to the occur of FRET. After adding of OTA aptamer and then introducing of OTA, the FRET would be interrupted/recovered due to the specific affinity of OTA and its aptamer, the fluorescence recovery value would increase with the addition of OTA. Under the optimal experimental conditions (pH 7, m_{GQD/nanoceria} 2, c_aptamer 100 nM, incubation time 30 min), the constructed ratiometric fluorescence aptasensor exhibited a satisfying linear range (0.01-20 ng mL^-1), low limit of detection (2.5 pg mL^-1) and good selectivity towards OTA, and has been successfully applied for the analysis of real sample peanuts with good accuracy of the recoveries ranged from 90 to 110%.

Keywords: Aptasensor; GQD; Nanoceria; Ochratoxin A; Ratiometric FRET.