Larkinella knui sp. nov., isolated from soil

Abstract

A Gram-stain-negative, motile by gliding, rod-shaped, aerobic bacterium, designated 15J6-3T6^T, was isolated from a soil sample collected from Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J6-3T6^T belongs to the family Cytophagaceae and is related to Larkinella harenae 15J9-9^T (93.9 % similarity), Larkinella arboricola Z0532^T (93.6 %), Larkinella bovis M2TB15^T (93.3 %), and Larkinella insperata LMG 22510^T (93.3 %). The DNA G+C content of strain 15J6-3T6^T was 50.6 mol%. The detection of phosphatidylethanolamine and an unidentified polar lipid as major polar lipids, menaquinone-7 as the predominant quinone, and C16 : 1ω5c, iso-C15 : 0, and iso-C17 : 0 3-OH as the major fatty acids also supports the affiliation of the isolate to the genus Larkinella. Based on its phenotypic properties and phylogenetic distinctiveness, we propose that strain 15J6-3T6^T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella knui sp. nov. is proposed. The type strain is 15J6-3T6^T (=KCTC 42998^T=JCM 31989^T).

Keywords: Bacteroidetes; Larkinella; soil bacteria.