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. 2018 Jan 5;17(1):6.
doi: 10.1186/s12936-017-2170-8.

Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar

Myat Htut Nyunt  1   2 , Than Naing Soe  3 , Thinzar Shein  2 , Ni Ni Zaw  2 , Soe Soe Han  2 , Fauzi Muh  1 , Seong-Kyun Lee  1 , Jin-Hee Han  1 , Ji-Hoon Park  1 , Kwon-Soo Ha  1   4 , Won Sun Park  1   5 , Seok-Ho Hong  1   6 , Myat Phone Kyaw  2 , Eun-Taek Han  7
Affiliations

Estimation on local transmission of malaria by serological approach under low transmission setting in Myanmar

Myat Htut Nyunt et al. Malar J. .

Abstract

Background: As the prevalence of the malaria has been decreasing in many endemic countries including Myanmar, malaria elimination in Greater Mekong Region was targeted not later than 2030. The relevance of molecular and serological tools to identify residual transmission remains to be established in this setting.

Methods: One-year cohort study was conducted and sera samples were collected in every 3 months with active and passive case detection for clinical malaria episodes by RDT, microscopy and molecular method. The sera were used to detect the malaria antibody against PfMSP1-19, PvAMA1, PvDBPII and PvMSP1-19 by protein microarray.

Results: Among the recruited 1182 participants, there was no RDT positive case for malaria infection although two vivax infections were detected by microscopy in initial collection. Molecular methods detected the asymptomatic cases of 28/1182 (2.37%) in first, 5/894 (0.42%) in second, 12/944 (1.02%) in third, 6/889 (0.51%) in fourth collection, respectively. Seropositivity rates against the PfMSP1-19, PvMSP1-19, PvAMA1 and PvDBPII were 73/270 (27.0%), 85/270 (31.5%), 65/270 (24.1%) and 160/270 (59.3%), respectively. PfMSP1-19 and PvMSP1-19 showed high and stable antigenicity in acute and subacute samples but declining in 1-year history samples. No cross reactivity of PfMSP1-19 and PvMSP1-19 between the two species and higher seropositivity among the asymptomatic carriers were observed. Mapping data indicated serological surveillance can detect the geographical pattern of malaria infection under low transmission setting.

Conclusions: These findings support that PfMSP1-19 and PvMSP1-19 are suggested for serosurveillance of the malaria especially in low transmission setting for further necessary actions have to be carried out to eliminate the malaria.

Keywords: Asymptomatic cases; Malaria; Myanmar; Serological surveillance.

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Figures

Fig. 1
Fig. 1
Selection of the sera for serological assessment among the participants
Fig. 2
Fig. 2
Seropositivity of the four different antigens among four visit (V1–V4)
Fig. 3
Fig. 3
Serokinetic of the vivax antigens among the asymptomatic vivax infected cases
Fig. 4
Fig. 4
Mapping of the seroprevalence of the four different targets and molecular confirmed subclinical cases. The size of the circle represents the relative percent of the positive cases
See this image and copyright information in PMC

References

    1. Elliott SR, Fowkes FJ, Richards JS, Reiling L, Drew DR, Beeson JG. Research priorities for the development and implementation of serological tools for malaria surveillance. F1000Prime Rep. 2014;6:100. doi: 10.12703/P6-100. - DOI - PMC - PubMed
    1. WHO . Malaria elimination. A field manual for low and moderate endemic countries. Geneva: World Health Organization; 2007.
    1. Sturrock HJW, Hsiang MS, Cohen JM, Smith DL, Greenhouse B, Bousema T, Gosling RD. Targeting asymptomatic malaria infections: active surveillance in control and elimination. PLoS Med. 2013;10:e1001467. doi: 10.1371/journal.pmed.1001467. - DOI - PMC - PubMed
    1. WHO . Policy brief on malaria diagnostics in low-transmission settings. Geneva: World Health Organization; 2014.
    1. Wipasa J, Suphavilai C, Okell LC, Cook J, Corran PH, Thaikla K. Long-lived antibody and B Cell memory responses to the human malaria parasites, Plasmodium falciparum and Plasmodium vivax. PLoS Pathog. 2010;6:e1000770. doi: 10.1371/journal.ppat.1000770. - DOI - PMC - PubMed

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