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. 1989 Apr 14;980(2):209-19.
doi: 10.1016/0005-2736(89)90401-x.

Synthesis and characterization of fluorescent Lucifer yellow-lipid conjugates

E A Nothnagel  1
Affiliations

Synthesis and characterization of fluorescent Lucifer yellow-lipid conjugates

E A Nothnagel. Biochim Biophys Acta. .

Abstract

The syntheses of fluorescent lipid probes composed of Lucifer yellow dyes linked to either cholesterol or phospholipids are described. The spectral properties of these probes are characterized, and the probes are evaluated for use with model membranes and with live animal and plant cells. Of the probes synthesized, the cholesterol derivative is the easiest to prepare and appears to be the most useful because it readily labels the plasma membrane of live cells and maintains a high ratio of cell surface-to-cytoplasmic fluorescence.

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