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. 2017 Dec 11:8:2457.
doi: 10.3389/fmicb.2017.02457. eCollection 2017.

Transmission Biology of Rice Stripe Mosaic Virus by an Efficient Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

Xin Yang  1 , Tong Zhang  1 , Biao Chen  1 , Guohui Zhou  1
Affiliations

Transmission Biology of Rice Stripe Mosaic Virus by an Efficient Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

Xin Yang et al. Front Microbiol. .

Abstract

Rice stripe mosaic virus (RSMV) is a newly discovered species of cytorhabdovirus infecting rice plants that is transmitted by the leafhopper Recilia dorsalis. In this study, the transmission characteristics of RSMV by R. dorsalis were investigated. Under suitable growth conditions for R. dorsalis, the RSMV acquisition rate reached 71.9% in the second-generation population raised on RSMV-infected rice plants. The minimum acquisition and inoculation access periods of R. dorsalis were 3 and 30 min, respectively. The minimum and maximum latent transmission periods of RSMV in R. dorsalis were 6 and 18 d, respectively, and some R. dorsalis intermittently transmitted RSMV at 2-6 d intervals. Our findings revealed that the virus can replicate in the leafhopper body, but is likely not transovarially transmitted to offspring. These transmission characteristics will help guide the formulation of RSMV prevention and control strategies.

Keywords: Recilia dorsalis; cytorhabdovirus; rice disease; rice stripe mosaic virus; transmission characteristics.

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Figures

Figure 1
Figure 1
Electron microscopy of RSMV in the midgut of R. dorsalis. Midgut tissues of viruliferous R. dorsalis adults were dissected and negative-stained. RSMV virions were observed using a transmission electron microscope. “VP”: viroplasm; black arrows: RSMV virions.
Figure 2
Figure 2
Relative RSMV titer in R. dorsalis after its acquisition from rice plants. Accumulation levels of the virus in R. dorsalis were normalized relative to the Actin gene at different time points as assessed by RT-qPCR. Each histogram bar represents the RSMV's relative mean titer from at least 30 individuals. Values followed by different letters represent significant differences based on a statistical analysis (SPSS 13.0) followed by Duncan's multiple range test at P < 0.05.
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