Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Editorial
. 2017 Dec;9(12):4867-4870.
doi: 10.21037/jtd.2017.11.73.

Optogenetic tools for cell biological applications

Abhirup Mukherjee  1 , Nicole A Repina  2   3 , David V Schaffer  2   4   5   6 , Ravi S Kane  1
Affiliations
Editorial

Optogenetic tools for cell biological applications

Abhirup Mukherjee et al. J Thorac Dis. 2017 Dec.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Representative schematic of the BphP1-Q-PAS1 system. Protein size is indicated by relative size of bars. Sample proteins A and B are drawn to a scale of 500 amino acids. (A) Light-induced reversible dimerization between BphP1 (red) and Q-PAS1 (beige) leads to interaction of fused domains (grey); (B) schematic showing domain structures of PpsR2 and Q-PAS1. Adapted with permission from Redchuk et al. (8).
Figure 2
Figure 2
Tridirectional subcellular translocation of protein and spectral multiplexing with blue and NIR-light using the AsLOV2 and BphP1-Q-PAS1 systems. (A) Schematic showing blue-light-induced nuclear translocation and NIR-light-induced plasma membrane translocation of attached protein, mCherry (red); (B) live fluorescence image showing sequential translocation of mCherry to the nucleus and plasma membrane in response to indicated illumination with intermittent dark relaxation periods. Adapted with permission from Redchuk et al. (8). NIR, near-infrared.
See this image and copyright information in PMC

Comment on

References

    1. Repina NA, Rosenbloom A, Mukherjee A, et al. At light speed: advances in optogenetic systems for regulating cell signaling and behavior. Annu Rev Chem Biomol Eng 2017;8:13-39. 10.1146/annurev-chembioeng-060816-101254 - DOI - PMC - PubMed
    1. Optogenetics Deisseroth K. Nat Methods 2011;8:26-9. 10.1038/nmeth.f.324 - DOI - PMC - PubMed
    1. Kennedy MJ, Hughes RM, Peteya LA, et al. Rapid blue light induction of protein interactions in living cells. Nat Methods 2010;7:973-5. 10.1038/nmeth.1524 - DOI - PMC - PubMed
    1. Bugaj LJ, Choksi AT, Mesuda CK, et al. Optogenetic protein clustering and signaling activation in mammalian cells. Nat Methods 2013;10:249-52. 10.1038/nmeth.2360 - DOI - PubMed
    1. Taslimi A, Vrana JD, Chen D, et al. An optimized optogenetic clustering tool for probing protein interaction and function. Nat Commun 2014;5:4925. 10.1038/ncomms5925 - DOI - PMC - PubMed