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. 2018 Mar 2;8(3):899-908.
doi: 10.1534/g3.117.300543.

Gene Identification of Pheromone Gland Genes Involved in Type II Sex Pheromone Biosynthesis and Transportation in Female Tea Pest Ectropis grisescens

Zhao-Qun Li  1 , Long Ma  2 , Qian Yin  3 , Xiao-Ming Cai  1 , Zong-Xiu Luo  1 , Lei Bian  1 , Zhao-Jun Xin  1 , Peng He  4 , Zong-Mao Chen  5
Affiliations

Gene Identification of Pheromone Gland Genes Involved in Type II Sex Pheromone Biosynthesis and Transportation in Female Tea Pest Ectropis grisescens

Zhao-Qun Li et al. G3 (Bethesda). .

Abstract

Moths can biosynthesize sex pheromones in the female sex pheromone glands (PGs) and can distinguish species-specific sex pheromones using their antennae. However, the biosynthesis and transportation mechanism for Type II sex pheromone components has rarely been documented in moths. In this study, we constructed a massive PG transcriptome database (14.72 Gb) from a moth species, Ectropis grisescens, which uses type II sex pheromones and is a major tea pest in China. We further identified putative sex pheromone biosynthesis and transportation-related unigenes: 111 cytochrome P450 monooxygenases (CYPs), 25 odorant-binding proteins (OBPs), and 20 chemosensory proteins (CSPs). Tissue expression and phylogenetic tree analyses showed that one CYP (EgriCYP341-fragment3), one OBP (EgriOBP4), and one CSP (EgriCSP10) gene displayed an enriched expression in the PGs, and that EgriOBP2, 3, and 25 are clustered in the moth pheromone-binding protein clade. We considered these our candidate genes. Our results yielded large-scale PG sequence information for further functional studies.

Keywords: Ectropis grisescens; sex pheromone biosynthesis; tissue expression pattern; transcriptome.

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Figures

Figure 1
Figure 1
Annotation summaries for E. grisescens unigenes. (A) Species distribution of unigenes with the best-hit annotation terms in the nonredundant (NR) database. (B) Gene ontology (GO) classifications of E. grisescens unigenes.
Figure 2
Figure 2
Phylogenetic analysis of CYPs in E. grisescens, O. brumata, and B. mori. The phylogenetic tree was constructed in PhyML3.0 using the maximum likelihood method.
Figure 3
Figure 3
Phylogenetic analysis of EgriOBPs with some other insect OBPs. The phylogenetic tree was constructed in PhyML3.0 using the maximum likelihood method.
Figure 4
Figure 4
Phylogenetic analysis of EgriCSPs with some other insect CSPs. The phylogenetic tree was constructed in PhyML3.0 using the maximum likelihood method.
Figure 5
Figure 5
Tissue expression profile of selected EgriCYP genes based on relative mRNA quantity and the FPKM values of EgriCYPs with PG-enriched expression. The level of EgriCYPs expression in the abdomen was set at 1. B, abdomen; Pg, female pheromone glands.
Figure 6
Figure 6
Tissue expression profile of selected EgriOBP genes based on relative mRNA quantity. The level of EgriOBPs expression in the female antennae was set at 1. A, antennae; Ab, abdomen; H, heads; L, legs; Pg, female pheromone glands; Pr, proboscises; T, thoraxes; W, wings.
Figure 7
Figure 7
Tissue expression profile of selected EgriCSP genes based on relative mRNA quantity. The level of EgriCSPs expression in the female antennae was set at 1. A, antennae; Ab, abdomen; H, heads; L, legs; Pg, female pheromone glands; Pr, proboscises; T, thoraxes; W, wings.
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