A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells

Andreas Kurtz¹, Stefanie Seltmann², Amos Bairoch³, Marie-Sophie Bittner⁴, Kevin Bruce⁵, Amanda Capes-Davis⁶, Laura Clarke⁷, Jeremy M Crook⁸, Laurence Daheron⁹, Johannes Dewender⁴, Adam Faulconbridge⁷, Wataru Fujibuchi¹⁰, Alexander Gutteridge¹¹, Derek J Hei¹², Yong-Ou Kim¹³, Jung-Hyun Kim¹³, Anja Kolb- Kokocinski¹⁴, Fritz Lekschas⁴, Geoffrey P Lomax¹⁵, Jeanne F Loring¹⁶, Tenneille Ludwig¹⁷, Nancy Mah⁴, Tohru Matsui¹⁸, Robert Müller⁴, Helen Parkinson⁷, Michael Sheldon¹⁹, Kelly Smith²⁰, Harald Stachelscheid²¹, Glyn Stacey²², Ian Streeter⁷, Anna Veiga²³, Ren-He Xu²⁴

Affiliations

¹ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany. Electronic address: andreas.kurtz@charite.de.
² Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany. Electronic address: stefanie.seltmann@charite.de.
³ CALIPHO group, University of Geneva and Swiss Institute of Bioinformatics, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland.
⁴ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany.
⁵ Roslin Cells Limited and EBiSC, Edinburgh BioQuarter, Edinburgh EH16 4UX, UK.
⁶ CellBank Australia, Children's Medical Research Institute (CMRI), Wentworthville, NSW 2145, Australia.
⁷ European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
⁸ ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, NSW 2519, Australia; Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia; Department of Surgery, St Vincent's Hospital, The University of Melbourne, Fitzroy, VIC 3065, Australia.
⁹ Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
¹⁰ Center for iPS Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
¹¹ Pfizer, Neuroscience and Pain RU, Portway, Granta Park, Cambridge CB21 6GP, UK.
¹² Waisman Biomanufacturing, Waisman Center, University of Wisconsin, 1500 Highland Avenue, Madison, WI 53705, USA.
¹³ Division of Intractable Diseases, Center for Biomedical Sciences, National Institute of Health and Korea Centers for Diseases Control and Prevention, Chungcheongbuk-do 363-951, Republic of Korea.
¹⁴ Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SD, UK.
¹⁵ California Institute for Regenerative Medicine, Lake Merritt Plaza, 1999 Harrison Street STE 1650, Oakland, CA 94612, USA.
¹⁶ Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road SP30-3021, La Jolla, CA 92037, USA.
¹⁷ WiCell Research Institute (WiCell Stem Cell Bank), Madison, WI 53719, USA.
¹⁸ Keio University School of Medicine, the Center for Medical Genetics, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
¹⁹ Department of Genetics, Rutgers, The State University of New Jersey, Life Sciences Building, Piscataway, NJ 08854-8009, USA.
²⁰ University of Massachusetts Medical School, International Stem Cell Registry, 55 Lake Avenue North, Worcester, MA 01655, USA.
²¹ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany; Berlin Institute of Health, Stem Cell Core Unit, Berlin 13353, Germany.
²² National Institute for Biological Standards and Control a Centre of the MHRA, South Mimms, South Mimms, Hertfordshire EN6 3QG, UK; International Stem Cell Banking Initiative, Barley, Hertfordshire EN6 3QG, UK.
²³ Barcelona Stem Cell Bank, Center of Regenerative Medicine in Barcelona, 08908 Hospitalet de Llobregat, Barcelona, Spain.
²⁴ Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China.

PMID: 29320760
PMCID: PMC5768986
DOI: 10.1016/j.stemcr.2017.12.002

Review

A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells

Andreas Kurtz et al. Stem Cell Reports. 2018.

. 2018 Jan 9;10(1):1-6.

doi: 10.1016/j.stemcr.2017.12.002.

Authors

Affiliations

¹ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany. Electronic address: andreas.kurtz@charite.de.
² Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany. Electronic address: stefanie.seltmann@charite.de.
³ CALIPHO group, University of Geneva and Swiss Institute of Bioinformatics, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland.
⁴ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany.
⁵ Roslin Cells Limited and EBiSC, Edinburgh BioQuarter, Edinburgh EH16 4UX, UK.
⁶ CellBank Australia, Children's Medical Research Institute (CMRI), Wentworthville, NSW 2145, Australia.
⁷ European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
⁸ ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, NSW 2519, Australia; Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia; Department of Surgery, St Vincent's Hospital, The University of Melbourne, Fitzroy, VIC 3065, Australia.
⁹ Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
¹⁰ Center for iPS Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
¹¹ Pfizer, Neuroscience and Pain RU, Portway, Granta Park, Cambridge CB21 6GP, UK.
¹² Waisman Biomanufacturing, Waisman Center, University of Wisconsin, 1500 Highland Avenue, Madison, WI 53705, USA.
¹³ Division of Intractable Diseases, Center for Biomedical Sciences, National Institute of Health and Korea Centers for Diseases Control and Prevention, Chungcheongbuk-do 363-951, Republic of Korea.
¹⁴ Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SD, UK.
¹⁵ California Institute for Regenerative Medicine, Lake Merritt Plaza, 1999 Harrison Street STE 1650, Oakland, CA 94612, USA.
¹⁶ Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road SP30-3021, La Jolla, CA 92037, USA.
¹⁷ WiCell Research Institute (WiCell Stem Cell Bank), Madison, WI 53719, USA.
¹⁸ Keio University School of Medicine, the Center for Medical Genetics, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
¹⁹ Department of Genetics, Rutgers, The State University of New Jersey, Life Sciences Building, Piscataway, NJ 08854-8009, USA.
²⁰ University of Massachusetts Medical School, International Stem Cell Registry, 55 Lake Avenue North, Worcester, MA 01655, USA.
²¹ Charité - Universitätsmedizin Berlin, Berlin-Brandenburg Center for Regenerative Therapies, Berlin 13353, Germany; Berlin Institute of Health, Stem Cell Core Unit, Berlin 13353, Germany.
²² National Institute for Biological Standards and Control a Centre of the MHRA, South Mimms, South Mimms, Hertfordshire EN6 3QG, UK; International Stem Cell Banking Initiative, Barley, Hertfordshire EN6 3QG, UK.
²³ Barcelona Stem Cell Bank, Center of Regenerative Medicine in Barcelona, 08908 Hospitalet de Llobregat, Barcelona, Spain.
²⁴ Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China.

PMID: 29320760
PMCID: PMC5768986
DOI: 10.1016/j.stemcr.2017.12.002

Abstract

Unambiguous cell line authentication is essential to avoid loss of association between data and cells. The risk for loss of references increases with the rapidity that new human pluripotent stem cell (hPSC) lines are generated, exchanged, and implemented. Ideally, a single name should be used as a generally applied reference for each cell line to access and unify cell-related information across publications, cell banks, cell registries, and databases and to ensure scientific reproducibility. We discuss the needs and requirements for such a unique identifier and implement a standard nomenclature for hPSCs, which can be automatically generated and registered by the human pluripotent stem cell registry (hPSCreg). To avoid ambiguities in PSC-line referencing, we strongly urge publishers to demand registration and use of the standard name when publishing research based on hPSC lines.

Keywords: cell data referencing; cell databases; cell line authentication; cell line nomenclature; human pluripotent stem cells; stem cell registry.

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Figures

**Figure 1**
Naming Scheme for hPSC Lines A donor is recruited and hPSC lines generated by diverse generators x, y, … n or deposited in banks and registries, which submit the essential name elements to hPSCreg to automatically generate a standard name. A BioSamples ID for the line and the donor is automatically provided in parallel for each cell line. The names and BioSamples IDs are locked and registered in a catalog in the hPSCreg database as well as in the Cellosaurus cell line database. Historical lines with non-standard names are often used in hPSC banks and registries. Registration of these lines in hPSCreg will automatically provide a standard name and link it to the pre-existing names, which remain as synonyms.

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References

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A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells

Affiliations

A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells

Authors

Affiliations

Abstract

Figures

References

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MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

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