Bioassay-guided isolation of novel and selective urease inhibitors from Diospyros lotus

Abstract

Two new dimeric naphthoquinones, 5',8'-dihydroxy-6,6'-dimethyl-7,3'-binaphthyl-1,4,1',4'-tetraone (1; Di-naphthodiospyrol D) and 5',8'-dihydroxy-5,8-dimethoxy-6,6'-dimethyl-7,3'-binaphthyl-1,4,1',4'-tetraone (2; Di-naphthodiospyrol E), along with known naphthoquinones diospyrin (3) and 8-hydroxy diospyrin (4) were isolated from the chloroform fraction of extract of Diospyros lotus roots. Their structures were elucidated by advanced spectroscopic analyses, including HSQC, HMBC, NOESY, and J-resolved NMR experiments. The fractions and compounds 1-4 were evaluated for urease activity and phosphodiesterase-I, carbonic anhydrase-II and α-chymotrypsin enzyme inhibitory activities. Compounds 1 and 2 and their corresponding fractions showed significant and selective inhibitory effects on urease activities. The IC₅₀ values of 1 and 2 were 260.4 ± 6.37 and 381.4 ± 4.80 µmol·L^-1, respectively, using thiourea (IC₅₀ = 21 ± 0.11 µmol·L^-1) as the standard inhibitor. This was the first report demonstrating that the naphthoquinones class showed urease inhibition.

Keywords: Carbonic anhydrase-II; Diospyros lotus; Naphthoquinones; Phosphodiesterase-I; Urease inhibitory activities; α-Chymotrypsin.