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Published Erratum
. 2018 Jan;30(1):249-252.
doi: 10.1105/tpc.17.00893. Epub 2018 Jan 12.

CORRECTION

No authors listed
Published Erratum

CORRECTION

No authors listed. Plant Cell. 2018 Jan.
No abstract available

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Figures

Figure 2.
Figure 2.
ROXY1 Interacts with TGA Factors in the Nuclei of Transiently Transformed N. benthamiana Leaves. The BiFC technique was employed to investigate ROXY1 interactions with TGA factors in planta. The N terminus of YFP (YN) was cloned in-frame upstream of ROXY1. The C terminus of YFP (YC) was cloned in-frame upstream of TGA2, TGA3, TGA7, and PAN individually. Constructs were pairwise transiently expressed in N. benthamiana leaves. Reconstitution of YFP fluorescence was examined by confocal microscopy 3 to 5 d after transient coexpression of protein pairs, and representative pictures are shown. Yellow fluorescence in the nucleus was detected for interactions of ROXY1 with PAN, TGA2, TGA3, and TGA7, respectively. As a negative control, coexpression of YN-ROXY1 with nonfused YC failed to reconstitute a fluorescent YFP chromophore. Bars = 50 μm.
Figure 6.
Figure 6.
Interaction between ROXY1 and PAN. (A) and (B) show typical petal phenotypes of roxy1-3 and roxy1-2 mutants, where petals remain often smaller or folded. The roxy1-2 pan double mutant (D) to (F) develops pentamerous flowers resembling those of the pan single mutant (C). Additionally, later petal morphogenesis is affected in double mutants that also form smaller (D) or folded petals in (D) to (F), thus resembling later roxy1-2 petal differentiation. The image shown in (A) is of a roxy1-3 mutant published in Xing et al. (2005), Figure 1C. The image shown in (B) was duplicated from Figure 1B, lower left, flipped horizontally.
Figure 7.
Figure 7.
Petal Phenotypes Induced by the Chimeric PAN Repressor. To overcome functional redundancy of TGA factors, PAN was fused to the EAR-motif repression domain (SRDX). Ectopic expression of PANSRDX induced severe defects during flower development (500 flowers, 10 flowers from 50 T1 plants, were examined). Floral phenotypes of 35Spro:PANSRDX T1 transgenic plants grouping into four distinct classes are shown. (A) Tetramerous wild-type-like flowers with four normal petals (10.6%). (B) Pentamerous flowers with five normal petals resembling those of the single pan mutant (19.0%). (C) and (D) Tetramerous flowers with normal, smaller, or folded petals resembled those of the single roxy1-2 mutant (19.6%). The majority of analyzed flowers was pentamerous (50.8%) and developed normal but also smaller (E) or folded petals (F), thus resembling those of the roxy1-2 pan double mutant. Abnormal petals, such as smaller or folded ones, are marked by arrows. The image shown in (A) was duplicated from Figure 3D, upper left.
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Erratum for

References

    1. Xing S., Rosso M.G., and Zachgo S. (2005). ROXY1, a member of the plant glutaredoxin family, is required for petal development in Arabidopsis thaliana. Development 132: 1555–1565. - PubMed

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