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. 1985;20(3):209-13.
doi: 10.1007/BF00205578.

Effect of melphalan in vitro on induction of murine suppressor T cells by ConA

R Ophir, S Ben-Efraim

Effect of melphalan in vitro on induction of murine suppressor T cells by ConA

R Ophir et al. Cancer Immunol Immunother. 1985.

Abstract

The effect of treatment with melphalan in vitro on the activity of spleen cells from BALB/c mice was investigated. Incubation of spleen cells with 1.5-5 micrograms melphalan/1 X 10(7) inhibited subsequent mitogenic stimulation by ConA or PHA and the allogeneic response of BALB/c spleen cells against C57B1 target spleen cells. Incubation of spleen cells with ConA led to induction of suppressor T cells which when added to fresh cultures inhibited the allogeneic response. Preincubation of spleen cells with melphalan even at low concentrations (0.15-0.5 micrograms 1 X 10(7) cells) which do not directly affect mitogenic stimulation or allogeneic response partially inhibited the generation of suppressor T cells by ConA. Treatment with melphalan had no effect on already induced suppressor T cells as shown by incubation of spleen cells with melphalan (0.15-5 micrograms/1 X 10(7) cells) after incubation with ConA. Addition of cells treated with melphalan alone (without ConA) to fresh cultures led to an increase in the allogeneic response.

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References

    1. Ben-Efraim S, Bocian RC, Mokyr MB, Dray S. Increase in the effectiveness of melphalan therapy with progression of MOPC-315 plasmacytomas tumor growth. Cancer Immunol Immunother. 1983;15:101. - PMC - PubMed
    1. Ben-Efraim S, Komlos L, Notmann J, Hart J, Halbrecht I. In vitro selective effect of melphalan on human T-cell populations. Cancer Immunol Immunother. 1985;19:53. - PMC - PubMed
    1. Bocian RC, Ben-Efraim S, Dray S, Mokyr MB. Melphalan-mediated potentiation of antitumor responsiveness of immunosuppressed spleen cells from mice bearing a large MOPC-315 tumor. Cancer Immunol Immunother. 1984;18:41. - PMC - PubMed
    1. Diamanstein T, Klos M, Hahn H, Kaufman SHE. Direct in vitro evidence for different susceptibilities to 4-hydroxyperoxycyclophosphamide of antigen-primed T cells regulating humoral and cell-mediated immune response to sheep erythrocytes: a possible explanation for the inverse action of cyclophosphamide on humoral and cell-mediated immune resonse. J Immunol. 1981;126:1717. - PubMed
    1. Dray S, Mokyr MB (1985) Immunomodulation by cyclophosphamide and its effect on eradication of established tumors. In: Fudenberg H, Ambrogy F (eds) Frontiers in Immunomodulation. Plenum Press New York, (in press)

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