Toxicokinetics of Zn and Cd in the earthworm Eisenia andrei exposed to metal-contaminated soils under different combinations of air temperature and soil moisture content

Abstract

This study evaluated how different combinations of air temperature (20 °C and 25 °C) and soil moisture content (50% and 30% of the soil water holding capacity, WHC), reflecting realistic climate change scenarios, affect the bioaccumulation kinetics of Zn and Cd in the earthworm Eisenia andrei. Earthworms were exposed for 21 d to two metal-contaminated soils (uptake phase), followed by 21 d incubation in non-contaminated soil (elimination phase). Body Zn and Cd concentrations were checked in time and metal uptake (k₁) and elimination (k₂) rate constants determined; metal bioaccumulation factor (BAF) was calculated as k₁/k₂. Earthworms showed extremely fast uptake and elimination of Zn, regardless of the exposure level. Climate conditions had no major impacts on the bioaccumulation kinetics of Zn, although a tendency towards lower k₁ and k₂ values was observed at 25 °C + 30% WHC. Earthworm Cd concentrations gradually increased with time upon exposure to metal-contaminated soils, especially at 50% WHC, and remained constant or slowly decreased following transfer to non-contaminated soil. Different combinations of air temperature and soil moisture content changed the bioaccumulation kinetics of Cd, leading to higher k₁ and k₂ values for earthworms incubated at 25 °C + 50% WHC and slower Cd kinetics at 25 °C + 30% WHC. This resulted in greater BAFs for Cd at warmer and drier environments which could imply higher toxicity risks but also of transfer of Cd within the food chain under the current global warming perspective.

Keywords: Bioaccumulation; Bioavailability; Climate change; Heavy metals; Mining wastes; Soil invertebrates.