Heparan sulphate proteoglycans and their polypeptide chains from BHK cells

Abstract

A simple procedure for partially purifying membrane-associated 35S-labelled proteoglycans from BHK cells is described. The labelled molecules are mainly heparan sulphate glycoproteins, and their average half-lives are approximately 3 h in culture. A new method, which depends on the degradation of heparan sulphate by nitrous acid at low pH, has been devised to determine the size of the polypeptide moiety of this class of molecule. The BHK cell heparan sulphate proteoglycans contain three main polypeptides having mol. wts. of approximately 65, 85 and 120 kd.