Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec;52(4):316-319.
doi: 10.5045/br.2017.52.4.316. Epub 2017 Dec 26.

Transplantation of human umbilical cord blood CD34+ cells into the liver of newborn NOD/SCID/IL-2Rγ null (NSG) mice after busulfan conditioning

Yunmi Ko  1 , Yeon Ho Jeong  2 , Jun Ah Lee  1   3
Affiliations

Transplantation of human umbilical cord blood CD34+ cells into the liver of newborn NOD/SCID/IL-2Rγ null (NSG) mice after busulfan conditioning

Yunmi Ko et al. Blood Res. 2017 Dec.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Authors' Disclosures of Potential Conflicts of Interest: No potential conflicts of interest relevant to this article were reported.

Figures

Fig. 1
Fig. 1. Survival and weight changes of newborn NSG mice after hCD34+ cell injection. Humanized NSG mice were monitored daily from the 3rd week of transplantation. Most of the newborn NSG mice did well, but two showed features suggesting graft versus host disease (weight loss, hunched posture, and diminished activity) and died on the 26th day of transplantation.
Fig. 2
Fig. 2. Reconstitution of human cells in newborn NSG mice after intrahepatic transplantation of CD34+ cells. Mononuclear cells derived from bone marrow, spleen, peripheral blood and liver tissues of humanized NSG mice were isolated in the 12th week of transplantation and were stained with anti-hCD45, anti-hCD3, and anti-hCD19 antibodies. Data are means±SEM and representative of four mice per group, excluding lowest values (N=5).
Abbreviations: BM, bone marrow; LV, liver; ND, not detected; PB, peripheral blood; SPL, spleen.
See this image and copyright information in PMC

References

    1. Shultz LD, Brehm MA, Garcia-Martinez JV, Greiner DL. Humanized mice for immune system investigation: progress, promise and challenges. Nat Rev Immunol. 2012;12:786–798. - PMC - PubMed
    1. Palis J, Robertson S, Kennedy M, Wall C, Keller G. Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse. Development. 1999;126:5073–5084. - PubMed
    1. Sasaki K, Iwatsuki H. Origin and fate of the central macrophages of erythroblastic islands in the fetal and neonatal mouse liver. Microsc Res Tech. 1997;39:398–405. - PubMed
    1. Martin MA, Bhatia M. Analysis of the human fetal liver hematopoietic microenvironment. Stem Cells Dev. 2005;14:493–504. - PubMed
    1. Dalakas E, Newsome PN, Harrison DJ, Plevris JN. Hematopoietic stem cell trafficking in liver injury. FASEB J. 2005;19:1225–1231. - PubMed

LinkOut - more resources