Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:7657190.
doi: 10.1155/2017/7657190. Epub 2017 Nov 29.

Purification, Characterization, and Mode of Action of Pentocin JL-1, a Novel Bacteriocin Isolated from Lactobacillus pentosus, against Drug-Resistant Staphylococcus aureus

Han Jiang  1 , Jiong Zou  1 , Hui Cheng  1 , Jiehong Fang  1 , Guangrong Huang  1
Affiliations

Purification, Characterization, and Mode of Action of Pentocin JL-1, a Novel Bacteriocin Isolated from Lactobacillus pentosus, against Drug-Resistant Staphylococcus aureus

Han Jiang et al. Biomed Res Int. 2017.

Abstract

Staphylococcus aureus and its drug-resistant strains, which threaten public health and food safety, are in need of effective control by biopreservatives. A novel bacteriocin, pentocin JL-1, produced by Lactobacillus pentosus that was isolated from the intestinal tract of Chiloscyllium punctatum, was purified by a four-step chromatographic process. Mass spectrometry based on MALDI-TOF indicated that pentocin JL-1 has a molecular mass of 2987.23 Da. Only six of the twenty-five amino acids could be identified by Edman degradation. This bacteriocin is thermostable and tolerates a pH range of 5-7. Also, it is sensitive to proteinase K, trypsin, pepsin, and alkaline protease. This bacteriocin has a broad inhibitory spectrum against both Gram-positive and Gram-negative strains and in particular is effective against multidrug-resistant S. aureus. Additionally, we showed that the cell membrane is the target of pentocin JL-1 against methicillin-resistant S. aureus (MRSA), causing a loss of proton motive force. Furthermore, pentocin JL-1 has a drastic impact on the structure and integrity of MRSA cells. These results suggest that pentocin JL-1 has potential as a biopreservative in the food industry.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Phylogenetic tree of strain JL-1 based on its 16S rRNA sequence.
Figure 2
Figure 2
Purification of the bacteriocin produced by L. pentosus JL-1 by semipreparative HPLC.
Figure 3
Figure 3
MALDI-TOF-MS of analytical HPLC purified pentocin JL-1.
Figure 4
Figure 4
Effects of pentocin JL-1 on intact cells. (a) The effects of pentocin JL-1 on MRSA GIM 1.771 growth and (b) time-killing kinetics by pentocin JL-1. Control (solid diamond); 1x MIC (solid triangle); 2x MIC (solid square); 3x MIC (solid star).
Figure 5
Figure 5
Analysis of ΔΨ of MRSA GIM1.771 cells. MRSA GIM 1.771 cells were treated with 3x MIC (a), 2x MIC (b), and 1x MIC (c) pentocin JL-1, respectively. 1% Triton X-100 was added as ΔΨ 100% dissipation and 0.05% (w/v) acetic acid was used as the negative control.
Figure 6
Figure 6
Analysis of ΔpH of MRSA GIM 1.771 cells. MRSA GIM 1.771 cells were treated with 3x MIC (solid star), 2x MIC (solid square), and 1x MIC (solid triangle) pentocin JL-1, respectively. 1% Triton X-100 (cross) was added as ΔpH 100% dissipation and 0.05% (w/v) acetic acid (solid diamond) was used as the negative control.
Figure 7
Figure 7
Scanning electron micrographs of MRSA GIM 1.771 cells. (a) Untreated control cells; (b) and (c) 1x MIC of pentocin JL-1 treated cells.
See this image and copyright information in PMC

References

    1. Rasooly R., Do P. M. In vitro cell-based assay for activity analysis of staphylococcal enterotoxin A in food: RESEARCH ARTICLE. FEMS Immunology & Medical Microbiology. 2009;56(2):172–178. doi: 10.1111/j.1574-695X.2009.00561.x. - DOI - PubMed
    1. Asao T., Kumeda Y., Kawai T., et al. An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: Estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiology and Infection. 2003;130(1):33–40. doi: 10.1017/S0950268802007951. - DOI - PMC - PubMed
    1. Ikeda T., Tamate N., Yamaguchi K., Makino S.-I. Quantitative analysis of Staphylococcus aureus in skimmed milk powder by real-time PCR. Journal of Veterinary Medical Science. 2005;67(10):1037–1041. doi: 10.1292/jvms.67.1037. - DOI - PubMed
    1. Fu X.-J., Fang Y., Yao M. Antimicrobial photodynamic therapy for methicillin-resistant Staphylococcus aureus infection. BioMed Research International. 2013;2013:9. doi: 10.1155/2013/159157.159157 - DOI - PMC - PubMed
    1. Culos K. A., Cannon J. P., Grim S. A. Alternative agents to vancomycin for the treatment of methicillin-resistant Staphylococcus aureus infections. American Journal of Therapeutics. 2013;20(2):200–212. doi: 10.1097/MJT.0b013e31821109ec. - DOI - PubMed

MeSH terms

LinkOut - more resources