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. 1985 Dec;164(3):1110-6.
doi: 10.1128/jb.164.3.1110-1116.1985.

Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu

K J Forbes, M C Pérombelon

Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu

K J Forbes et al. J Bacteriol. 1985 Dec.

Abstract

Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca. 10(-5) chromosomal recombinants per donor, the presence of a Mu prophage on the chromosome of the donor increased the frequency of mobilization of markers adjacent to the prophage by up to 10-fold. Using this system it was possible to order 17 chromosomal mutations. The behavior of Mu in E. carotovora subsp. carotovora was also studied.

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References

    1. Microbiol Rev. 1983 Sep;47(3):410-53 - PubMed
    1. Plasmid. 1978 Sep;1(4):584-8 - PubMed
    1. J Bacteriol. 1984 May;158(2):764-6 - PubMed
    1. J Gen Microbiol. 1982 Sep;128(9):1969-80 - PubMed
    1. J Bacteriol. 1975 Jan;121(1):320-31 - PubMed

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