Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan 15;15(1):11.
doi: 10.1186/s12985-018-0923-9.

Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes

Hao Wang  1   2 , Shouxin Li  3   4 , Asif Mahmood  1 , Shixing Yang  1 , Xiaochun Wang  1 , Quan Shen  1 , Tongling Shan  5 , Xutao Deng  6 , Jingjiao Li  7 , Xiuguo Hua  7 , Li Cui  7 , Eric Delwart  6 , Wen Zhang  8
Affiliations

Plasma virome of cattle from forest region revealed diverse small circular ssDNA viral genomes

Hao Wang et al. Virol J. .

Abstract

Background: Free-range cattle are common in the Northeast China area, which have close contact with farmers and may carry virus threatening to cattle and farmers.

Methods: Using viral metagenomics we analyzed the virome in plasma samples collected from 80 cattle from the forested region of Northeast China.

Results: The virome of cattle plasma is composed of the viruses belonging to the families including Parvoviridae, Papillomaviridae, Picobirnaviridae, and divergent viral genomes showing sequence similarity to circular Rep-encoding single stranded (CRESS) DNA viruses. Five such CRESS-DNA genomes were full characterized, with Rep sequences related to circovirus and gemycircularvirus. Three bovine parvoviruses belonging to two different genera were also characterized.

Conclusion: The virome in plasma samples of cattle from the forested region of Northeast China was revealed, which further characterized the diversity of viruses in cattle plasma.

Keywords: CRESS-DNA virus; Cattle blood; Parvovirus; Virome.

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
The genomic organization of the five CRESS-DNA viruses identified in the cattle blood samples
Fig. 2
Fig. 2
Phylogenetic analysis of the five CRESS-DNA viruses identified in the cattle blood samples. Phylogenetic analysis was performed based on the amino acid sequence of Rep protein. The sequence alignments included five CRESS-DNA viruses identified in the cattle blood samples in this study, their best BLASTp matches in GenBank based on the Rep proteins, and the representative strains of circovirus, cyclovirus, and gemycircularvirus. Host or sources of the closely related viruses of the five CRESS-DNA viruses in this study in the phylogenetic analysis were showed on branches. Viruses identified in this study were labeled with black dots
Fig. 3
Fig. 3
The genomic organization of the three BPVs and phylogenetic trees based on the nonstructural (NS) and VP1 proteins, respectively. a The genomic organization of the three BPVs identified in the cattle blood samples. b and (c) Phylogenetic analysis based on the amino acid sequence of NS and VP proteins, respectively. The sequence alignments included three BPVs identified in the cattle blood samples in this study, their best BLASTp matches in GenBank, and the representative members of the related genera in family Parvoviridae. Viruses identified in this study were labeled with black dots
See this image and copyright information in PMC

References

    1. Delwart E, Li L. Rapidly expanding genetic diversity and host range of the Circoviridae viral family and other rep encoding small circular ssDNA genomes. Virus Res. 2012;164:114–121. doi: 10.1016/j.virusres.2011.11.021. - DOI - PMC - PubMed
    1. Rosario K, Duffy S, Breitbart M. A field guide to eukaryotic circular single-stranded DNA viruses: insights gained from metagenomics. Arch Virol. 2012;157:1851–1871. doi: 10.1007/s00705-012-1391-y. - DOI - PubMed
    1. Zhang W, Li L, Deng X, Blümel J, Nübling CM, Hunfeld A, Baylis SA, Delwart E. Viral nucleic acids in human plasma pools. Transfusion. 2016;56:2248–2255. doi: 10.1111/trf.13692. - DOI - PubMed
    1. Dunlap DS, Ng TFF, Rosario K, Barbosa JG, Greco AM, Breitbart M, Hewson I. Molecular and microscopic evidence of viruses in marine copepods. Proc Natl Acad Sci. 2013;110:1375–1380. doi: 10.1073/pnas.1216595110. - DOI - PMC - PubMed
    1. Ng T, Alavandi S, Varsani A, Burghart S, Breitbart M. Metagenomic identification of a nodavirus and a circular ssDNA virus in semi-purified viral nucleic acids from the hepatopancreas of healthy Farfantepenaeus Duorarum shrimp. Dis Aquat Org. 2013;105:237–242. doi: 10.3354/dao02628. - DOI - PubMed

Publication types