Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan 16;8(1):809.
doi: 10.1038/s41598-017-18682-3.

Zika Virus in Salivary Glands of Five Different Species of Wild-Caught Mosquitoes from Mexico

Darwin Elizondo-Quiroga  1 , Aarón Medina-Sánchez  2 , Jorge M Sánchez-González  2 , Kristen Allison Eckert  3 , Erendira Villalobos-Sánchez  1 , Antonio Rigoberto Navarro-Zúñiga  2 , Gustavo Sánchez-Tejeda  4 , Fabián Correa-Morales  4 , Cassandra González-Acosta  4 , Carlos F Arias  5 , Susana López  5 , Rosa María Del Ángel  6 , Victoria Pando-Robles  7 , Armando E Elizondo-Quiroga  8
Affiliations

Zika Virus in Salivary Glands of Five Different Species of Wild-Caught Mosquitoes from Mexico

Darwin Elizondo-Quiroga et al. Sci Rep. .

Erratum in

Abstract

Zika virus (ZIKV) is a mosquito-borne pathogen, and Aedes aegypti has been identified as the main vector of the disease. Other mosquito species in the Aedes and Culex genera have been suggested to have the potential for being competent vectors based on experimental exposition of mosquitoes to an infectious blood meal containing ZIKV. Here, we report the isolation in cell culture of ZIKV obtained from different body parts of wild-caught female mosquitoes (Ae. aegypti, Ae. vexans, Cx. quinquefasciatus, Cx. coronator, and Cx. tarsalis) and whole male mosquitoes (Ae. aegypti and Cx. quinquefasciatus) in Mexico. Importantly, this is the first report that shows the presence of the virus in the salivary glands of the wild-caught female mosquitoes species, Cx. coronator, Cx. tarsalis, and Ae. vexans. Our findings strongly suggest that all the species reported herein are potential vectors for ZIKV.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Map of the collection points in the metropolitan area of Guadalajara, Jalisco, Mexico. Maps were generated using the free and open source software QGIS Chugiak version 2.4.0 (http://www.qgis.org/es/site/about/index.html).
Figure 2
Figure 2
Flow chart of mosquito collection in the metropolitan area of Guadalajara, Jalisco, Mexico. The figure outlines the collection municipalities and their neighborhoods, as well as the mosquitoes collected and the pools assembled for each area. The neighborhood El Vergel was the only area with mosquitoes positive for ZIKV. Also presented is the minimum infection rate (MIR) per species and the confidence intervals. F = Female; M = Male; ZIKV = Zika Virus.
Figure 3
Figure 3
Cytopathic effect in infected and uninfected cell cultures. Cytopathic effects consisting of cell rounding, detachment and culture degeneration were observed in C6/36 and Vero cell lines at day 4 post-ZIKV inoculation, under an inverted microscope (original magnification ×20 in C6/36 and ×10 in Vero cells. Scale bars, 100 μm). (A) Uninfected C6/36 cells, (B) C6/36 cells inoculated with salivary glands homogenates showing CPE, (C) Uninfected Vero cells, (D) Vero cells inoculated with salivary glands homogenates showing CPE.
See this image and copyright information in PMC

References

    1. Zanluca C, et al. First report of autochthonous transmission of Zika virus in Brazil. Mem Inst Oswaldo Cruz. 2015;110:569–572. doi: 10.1590/0074-02760150192. - DOI - PMC - PubMed
    1. CDC. Centers for Disease Control and Prevention, all countries and territories with active Zika virus transmission., (https://www.cdc.gov/zika/geo/active-countries.html) (2017).
    1. Secretaría de Salud (Mexico). Avisos epidemiológicos Zika., (https://www.gob.mx/cms/uploads/attachment/file/258140/Cuadro_Casos_ZIKA_...) (2017).
    1. Li MI, Wong PSJ, Ng LC, Tan CH. Oral susceptibility of Singapore Aedes (Stegomyia) aegypti (Linnaeus) to Zika virus. PLoS Negl Trop Dis. 2012;6:e1792. doi: 10.1371/journal.pntd.0001792. - DOI - PMC - PubMed
    1. Costa-da-Silva AL, et al. Laboratory strains of Aedes aegypti are competent to Brazilian Zika virus. PloS one. 2017;12:e0171951. doi: 10.1371/journal.pone.0171951. - DOI - PMC - PubMed

Publication types