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. 2018 Jan 11;51(3):e6955.
doi: 10.1590/1414-431X20176955.

Time collection and storage conditions of lipid profile

C N França  1   2 , C C Mendes  3 , C E S Ferreira  3   4
Affiliations

Time collection and storage conditions of lipid profile

C N França et al. Braz J Med Biol Res. .

Abstract

The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.

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Figures

Figure 1.
Figure 1.. Total cholesterol (A), triglycerides (B), low-density lipoprotein cholesterol - LDL-C (C), high-density lipoprotein cholesterol (HDL-C; D) and non-HDL-C (E) were analyzed at three different conditions: within 1 h after collection, after 2 to 3 h in the refrigerator and after 4 to 5 h at room temperature. Data are reported as medians and interquartile ranges. There were no significant differences among groups at the conditions evaluated (ANOVA).
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