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Case Reports
. 2018 Jun;183(3):623-627.
doi: 10.1007/s11046-018-0242-0. Epub 2018 Jan 16.

Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis

Rui Kano  1 , Yun-Hsia Hsiao  2 , Hock Siew Han  3 , Charles Chen  2 , Atsuhiko Hasegawa  4 , Hiroshi Kamata  5
Affiliations
Case Reports

Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis

Rui Kano et al. Mycopathologia. 2018 Jun.

Erratum in

Abstract

To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2-4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was < 0.75. The additive effect of the efflux blocker FK506 on TRF resistance was detected in the TRF-resistant strain. These results indicated that the TRF resistance in this strain reflects overexpression of genes encoding ABC transporter proteins.

Keywords: ABC transporter; FK506; Microsporum canis; Resistance; Terbinafine.

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