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. 2018 Jan;23(1):1-4.
doi: 10.1117/1.JBO.23.1.010502.

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng-Syong Wu  1   2 , Yung-Chin Chung  1 , Jun-Jei Chien  1 , Chien Chou  1   3
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Free article

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng-Syong Wu et al. J Biomed Opt. 2018 Jan.
Free article

Abstract

A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

Keywords: aberrations; confocal fluorescence microscopy; optical heterodyne.

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