Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2018 Jan 18:83:12.2.1-12.2.14.
doi: 10.1002/cpcy.31.

Basics of Digital Microscopy

Callen T Wallace  1 , Morgan Jessup  1 , Tytus Bernas  2 , Karina A Peña  1 , Michael J Calderon  1 , Patricia A Loughran  1
Affiliations
Review

Basics of Digital Microscopy

Callen T Wallace et al. Curr Protoc Cytom. .

Abstract

Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, characteristics of image file formats, and data management. Further suggestions are given which serve, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging. © 2018 by John Wiley & Sons, Inc.

Keywords: compression; image file format; quantization; resolution; sampling; signal-to-noise ratio.

PubMed Disclaimer

References

Literature Cited

    1. Bernas, T. (2005). Basics of digital microscopy. Current Protocols in Cytometry, 31, 12.2:12.2.1-12.2.14. doi: 10.1002/0471142956.cy1202s31.
    1. Gonzalez, R. C. , & Woods, R. F. (2002). Digital image processing (2nd ed.). Upper Saddle River, NJ.: Prentice Hall.
    1. Houston, W. V. (1926). The fine structure and wave-length of the Balmer Lines. The Astrophysical Journal, 64, 81. doi: 10.1086/142989.
    1. Joubert, J. , & Sharma, D. (2011). Light microscopy digital imaging. Current Protocols in Cytometry, 58, 2.3:2.3.1-2.3.11. doi: 10.1002/0471142956.cy0203s58.
    1. Rayleigh, J. W. S. (1879). Investigations in Optics with special reference to the Spectroscope. Philosophical Magazine, 8, 261-274. doi: 10.1080/14786447908639684.

MeSH terms

LinkOut - more resources