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Guideline
. 2018 Apr 1;314(4):H724-H732.
doi: 10.1152/ajpheart.00512.2017. Epub 2018 Jan 5.

Guidelines for authors and reviewers on antibody use in physiology studies

Heddwen L Brooks  1 , Merry L Lindsey  2   3
Affiliations
Guideline

Guidelines for authors and reviewers on antibody use in physiology studies

Heddwen L Brooks et al. Am J Physiol Heart Circ Physiol. .

Abstract

Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/ .

Keywords: antibody; immunoblot analysis; immunoblotting; immunohistochemistry; rigor and reproducibility.

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Figures

Fig. 1.
Fig. 1.
Literature assessment of antibody (Ab) use in articles published in the American Journal of Physiology-Heart and Circulatory Physiology. A: Ab use in immunoblot experiments. B: Ab use in immunohistochemistry experiments. amt, amount; MW, molecular weight.
Fig. 2.
Fig. 2.
Sample laboratory notebook page for recording immunoblot experiments. Ab, antibody; PVDF, polyvinylidene difluoride.
See this image and copyright information in PMC

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