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. 2018 Jan 19;14(1):20.
doi: 10.1186/s12917-018-1334-2.

Vaccine resistant pseudorabies virus causes mink infection in China

Gui-Sheng Wang  1   2 , Yijun Du  3 , Jia-Qiang Wu  3 , Fu-Lin Tian  1 , Xue-Jie Yu  4 , Jin-Bao Wang  5
Affiliations

Vaccine resistant pseudorabies virus causes mink infection in China

Gui-Sheng Wang et al. BMC Vet Res. .

Abstract

Background: Pseudorabies, a highly contagious infectious disease of swine is caused by pseudorabies virus (PRV). PRV can cause fatal infection in other animal species.

Results: We report a deadly outbreak of pseudorabies that killed 87.2% (3522/4028) minks in a farm in 2014 in Shandong Province, China. PRV was isolated by using Vero cell culture and detected in mink samples by PCR from minks died during the outbreak. Epidemiological analysis indicated that 5.8% of minks (33/566) were PCR positive to PRV in Shandong Province. Phylogenetic analysis indicated that the PRV strains isolated from minks in this study were in the same clade with the Chinese porcine PRV isolates, which are resistant to the PRV vaccine.

Conclusions: We demonstrated that pseudorabies virus caused an outbreak of minks in a farm in Shandong Province of China and the virus has a very high infection rate in minks in Shandong Province, which is a challenge for the fur industry in China.

Keywords: China; Herpesvirus; Minks; Pseudorabies; Pseudorabies virus.

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Conflict of interest statement

Ethics approval

The animal experiments were approved by the Bioethic Committee of Shandong University (Permit Number: SYXK (Shandong Province) 2014 0011). This study was conducted in strict accordance with the rules of the Bioethic Committee of Shandong University. The farmers participated in this study provided oral informed consent.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Geographic location of Shandong Province of China (left) and the mink sample collection sites (right). Mink samples were collected from 14 (grey areas) of 17 cities in Shandong Province. The maps were drawn using the R Project for Statistical Computing (https://www.r-project.org/)
Fig. 2
Fig. 2
DNA sequence alignment of gD gene of mink isolates of PRV W-MPRV1 and W-MPRV2. W-MPRV1 had a deletion of 281 nucleotides from 787 nucleotide to 1069 nucleotide
Fig. 3
Fig. 3
Amino acid sequence alignment of gD of mink isolates of PRV W-MPRV1 and W-MPRV2. W-MPRV1 had a deletion of 93 amino acids near the C-terminal
Fig. 4
Fig. 4
Phylogenetic tree of Pseudorabies virus. The phylogenetic tree was constructed with gB gene sequence (left) and the concatenated sequence of gB, gC, gD, gE, gH and TK genes (right) using MEGA5 software with 1000 replicates for bootstrap testing. Numbers (> 50) above or below branches are posterior node probabilities. The GenBank number was labeled on each line. Dots indicated sequences obtained in this study. Scale bar indicates nucleotide substitutions per site
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