Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Sep;146(3):381-385.
doi: 10.4103/ijmr.IJMR_225_16.

Development & standardization of an in-house IgM indirect ELISA for the detection of parvovirus B19 infections

Kumaran Vadivel  1 , Mageshbabu Ramamurthy  1 , Sathish Sankar  1 , Amita Jain  2 , Padma Srikanth  3 , Asit Ranjan Ghosh  4 , Balaji Nandagopal  1 , Aravindan Nair  1 , Gopalan Sridharan  1
Affiliations

Development & standardization of an in-house IgM indirect ELISA for the detection of parvovirus B19 infections

Kumaran Vadivel et al. Indian J Med Res. 2017 Sep.

Abstract

Background & objectives: Parvovirus B19 infections occur worldwide; the infection is acquired early in childhood but could occur later. B19 is reported to cause infection in childhood febrile illnesses, and arthropathies in adults and children and in end-stage renal disease (ESRD) seen in adults. This study was designed to develop an in-house IgM indirect ELISA for serological screening among patients and controls, and to compare ELISA results with those of nested polymerase chain reaction (nPCR) assay.

Methods: An in-house IgM indirect ELISA was standardized using peptide sequence of VP1/VP2 region of parvovirus B19. A total of 201 children and adult with febrile illnesses, 216 individuals with non-traumatic arthropathies, 201 cases of chronic anaemia associated with ESRD and 100 healthy controls were tested. Serum was separated from the blood and subsequently used for DNA extraction. The nested polymerase chain reaction (nPCR) for the detection of B19V DNA was performed using primers targeting the overlapping region of VP1/VP2 capsid protein genes.

Results: A total of 618 samples were tested for parvovirus B19 by an in-house IgM indirect ELISA. Among these samples, six were positive by in-house ELISA. The inter-rater agreement between ELISA and PCR assays was calculated using kappa coefficient analysis. The value of κ was 0.77 and the strength of agreement was 'good' (P<0.001).

Interpretation & conclusions: The in-house IgM indirect ELISA was found to be simple with high sensitivity and specificity when compared with nPCR and could be used as an alternative to expensive commercial kits in resource-poor settings.

PubMed Disclaimer

Conflict of interest statement

Conflicts of Interest: None.

Similar articles

See all similar articles

References

    1. Cotmore SF, Tattersall P. Characterization and molecular cloning of a human parvovirus genome. Science. 1984;226:1161–5. - PubMed
    1. Ozawa K, Young N. Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures. J Virol. 1987;61:2627–30. - PMC - PubMed
    1. Anderson LJ, Tsou C, Parker RA, Chorba TL, Wulff H, Tattersall P, et al. Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay. J Clin Microbiol. 1986;24:522–6. - PMC - PubMed
    1. Reid DM, Reid TM, Brown T, Rennie JA, Eastmond CJ. Human parvovirus-associated arthritis: A clinical and laboratory description. Lancet. 1985;1:422–5. - PubMed
    1. Schmidt M, Themann A, Drexler C, Bayer M, Lanzer G, Menichetti E, et al. Blood donor screening for parvovirus B19 in Germany and Austria. Transfusion. 2007;47:1775–82. - PubMed

MeSH terms

Substances