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. 2018 Mar:146:13-15.
doi: 10.1016/j.mimet.2018.01.005. Epub 2018 Feb 3.

Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA

Satoshi Hasegawa  1 , Toru Jojima  1 , Masayuki Inui  2
Affiliations

Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA

Satoshi Hasegawa et al. J Microbiol Methods. 2018 Mar.

Abstract

An efficient method to construct xenogeneic genomic libraries with low errors and bias by circumventing restriction-modification systems that restrict methylated DNA was developed. Un-methylated genomic DNA of Escherichia coli prepared by ϕ29 DNA polymerase was introduced to Corynebacterium glutamicum R after ligation with un-methylated vector plasmids.

Keywords: Genomic library; Restriction-modification systems; Un-methylated DNA; Xenogeneic methylated DNA.

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