Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan 23;13(1):e0188768.
doi: 10.1371/journal.pone.0188768. eCollection 2018.

Characterization of plasmids harboring blaCTX-M and blaCMY genes in E. coli from French broilers

Fabrice Touzain  1   2 , Laetitia Le Devendec  1   2 , Claire de Boisséson  1   2 , Sandrine Baron  1   2 , Eric Jouy  1   2 , Agnès Perrin-Guyomard  2   3 , Yannick Blanchard  1   2 , Isabelle Kempf  1   2
Affiliations

Characterization of plasmids harboring blaCTX-M and blaCMY genes in E. coli from French broilers

Fabrice Touzain et al. PLoS One. .

Abstract

Resistance to extended-spectrum cephalosporins (ESC) is a global health issue. The aim of this study was to analyze and compare plasmids coding for resistance to ESC isolated from 16 avian commensal and 17 avian pathogenic Escherichia coli (APEC) strains obtained respectively at slaughterhouse or from diseased broilers in 2010-2012. Plasmid DNA was used to transform E. coli DH5alpha, and the resistances of the transformants were determined. The sequences of the ESC-resistance plasmids prepared from transformants were obtained by Illumina (33 plasmids) or PacBio (1 plasmid). Results showed that 29 of these plasmids contained the blaCTX-M-1 gene and belonged to the IncI1/ST3 type, with 27 and 20 of them carrying the sul2 or tet(A) genes respectively. Despite their diverse origins, several plasmids showed very high percentages of identity. None of the blaCTX-M-1-containing plasmid contained APEC virulence genes, although some of them were detected in the parental strains. Three plasmids had the blaCMY-2 gene, but no other resistance gene. They belonged to IncB/O/K/Z-like or IncFIA/FIB replicon types. The blaCMY-2 IncFIA/FIB plasmid was obtained from a strain isolated from a diseased broiler and also containing a blaCTX-M-1 IncI1/ST3 plasmid. Importantly APEC virulence genes (sitA-D, iucA-D, iutA, hlyF, ompT, etsA-C, iss, iroB-E, iroN, cvaA-C and cvi) were detected on the blaCMY-2 plasmid. In conclusion, our results show the dominance and high similarity of blaCTX-M-1 IncI1/ST3 plasmids, and the worrying presence of APEC virulence genes on a blaCMY-2 plasmid.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

References

    1. ANSES. Résapath—Réseau d'épidémiosurveillance de l'antibiorésistance des bactéries pathogènes animales, bilan 2015, Lyon et Ploufragan-Plouzané, France, novembre 2016, rapport, 152p. Maisons-Alfort; 2016. French
    1. EFSA-ECDC. EU Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2014. EFSA J. 2016;14(2):4380. - PMC - PubMed
    1. Girlich D, Poirel L, Carattoli A, Kempf I, Lartigue MF, Bertini A, et al. Extended-spectrum beta-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France. Applied Environ Microbiol. 2007;73(14):4681–5. - PMC - PubMed
    1. Chauvin C, Le Devendec L, Jouy E, Le Cornec M, Francart S, Marois-Crehan C, et al. National prevalence of resistance to third-generation cephalosporins in Escherichia coli isolates from layer flocks in France. Antimicrob Agents Chemother. 2013;57(12):6351–3. doi: 10.1128/AAC.01460-13 - DOI - PMC - PubMed
    1. Kojima A, Ishii Y, Ishihara K, Esaki H, Asai T, Oda C, et al. Extended-spectrum-β-lactamase-producing Escherichia coli strains isolated from farm animals from 1999 to 2002: Report from the Japanese veterinary antimicrobial resistance monitoring program. Antimicrobial Agents Chemother. 2005;49(8):3533–7. - PMC - PubMed

Publication types