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. 2018:1730:239-246.
doi: 10.1007/978-1-4939-7592-1_16.

Exploratory GC/MS-Based Metabolomics of Body Fluids

Carole Migné  1 , Stéphanie Durand  1 , Estelle Pujos-Guillot  2
Affiliations

Exploratory GC/MS-Based Metabolomics of Body Fluids

Carole Migné et al. Methods Mol Biol. 2018.

Abstract

GC/MS-based metabolomics is a powerful tool for metabolic phenotyping and biomarker discovery from body biofluids. In this chapter, we describe an untargeted metabolomic approach for plasma/serum and fecal water sample profiling. It describes a multistep procedure, from sample preparation, oximation/silylation derivatization, and data acquisition using GC/QToF to data processing consisting in data extraction and identification of metabolites.

Keywords: Data processing; Gas chromatography; Mass spectrometry; Untargeted metabolomics.

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