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. 1986 Feb 1;35(3):493-7.
doi: 10.1016/0006-2952(86)90225-x.

The effect of the sesquiterpene lactones from Geigeria on glycolytic enzymes

A R Gaspar, D J Potgieter, N M Vermeulen

The effect of the sesquiterpene lactones from Geigeria on glycolytic enzymes

A R Gaspar et al. Biochem Pharmacol. .

Abstract

The effect of sesquiterpene lactones isolated from Geigeria was tested on three glycolytic enzymes. Phosphofructokinase was inhibited irreversibly by all of the sesquiterpene lactones, with ivalin(III) giving the highest extent of inhibition. Values for the kinetic constants Ki (1.3 mM) and kp (2.2 min-1) were established. Hexokinase and glyceraldehyde-3-phosphate dehydrogenase were also strongly inhibited at 1 mM and 3 mM concentrations of sesquiterpene lactones, respectively. Pre-incubation of ivalin with dithiothreitol decreased its inhibiting effect on phosphofructokinase, hexokinase and glyceraldehyde-3-phosphate dehydrogenase activities. Phosphofructokinase and hexokinase were protected against inhibition by ivalin by their respective substrates, adenosine-5'-triphosphate and glucose.

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