Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase
- PMID: 293663
- PMCID: PMC411693
- DOI: 10.1073/pnas.76.11.5582
Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase
Abstract
An adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+.calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of of Ca2+.calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+.calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggest that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.
Similar articles
-
GTP is not required for calmodulin stimulation of bovine brain adenylate cyclase.Proc Natl Acad Sci U S A. 1982 Mar;79(5):1462-5. doi: 10.1073/pnas.79.5.1462. Proc Natl Acad Sci U S A. 1982. PMID: 6951189 Free PMC article.
-
Properties of detergent-dispersed adenylate cyclase from cerebral cortex. Presence of an inhibitor protein.J Neurochem. 1981 Sep;37(3):558-66. doi: 10.1111/j.1471-4159.1982.tb12523.x. J Neurochem. 1981. PMID: 6268748
-
Purification of the calmodulin-sensitive adenylate cyclase from bovine cerebral cortex.Biochemistry. 1985 Jul 2;24(14):3776-83. doi: 10.1021/bi00335a054. Biochemistry. 1985. PMID: 4041438
-
Calmodulin regulation of adenylate cyclase activity.Cell Calcium. 1985 Jun;6(3):213-6. doi: 10.1016/0143-4160(85)90007-7. Cell Calcium. 1985. PMID: 3893727 Review.
-
Purification of the catalytic subunit of adenylate cyclase in vertebrates: state of the art in 1987.Biochimie. 1987 Apr;69(4):263-9. doi: 10.1016/0300-9084(87)90016-2. Biochimie. 1987. PMID: 3115310 Review.
Cited by
-
GTP is not required for calmodulin stimulation of bovine brain adenylate cyclase.Proc Natl Acad Sci U S A. 1982 Mar;79(5):1462-5. doi: 10.1073/pnas.79.5.1462. Proc Natl Acad Sci U S A. 1982. PMID: 6951189 Free PMC article.
-
Ca2+/calmodulin sensitivity may be common to all forms of neural adenylate cyclase.Proc Natl Acad Sci U S A. 1989 Dec;86(23):9564-8. doi: 10.1073/pnas.86.23.9564. Proc Natl Acad Sci U S A. 1989. PMID: 2594785 Free PMC article.
-
Differential effects of Ca2+-calmodulin on adenylate cyclase activity cyclase activity in mouse and rat pancreatic islets.Biochem J. 1982 Jul 15;206(1):97-102. doi: 10.1042/bj2060097. Biochem J. 1982. PMID: 6751327 Free PMC article.
-
Properties of the separated catalytic and regulatory units of brain adenylate cyclase.Proc Natl Acad Sci U S A. 1980 Nov;77(11):6344-8. doi: 10.1073/pnas.77.11.6344. Proc Natl Acad Sci U S A. 1980. PMID: 6935648 Free PMC article.
-
Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.Mol Cell Biochem. 1981 Jul 7;37(2):75-90. doi: 10.1007/BF02354931. Mol Cell Biochem. 1981. PMID: 6268961 No abstract available.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
