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. 1979 Nov;76(11):5582-6.
doi: 10.1073/pnas.76.11.5582.

Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase

W A Toscano Jr, K R Westcott, D C LaPorte, D R Storm

Evidence for a dissociable protein subunit required for calmodulin stimulation of brain adenylate cyclase

W A Toscano Jr et al. Proc Natl Acad Sci U S A. 1979 Nov.

Abstract

An adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.1] preparation that is not stimulated by NaF,5'-guanylyl imidodiphosphate, or Ca2+.calmodulin has been isolated from bovine cerebral cortex by Affi-Gel Blue chromatography and calmodulin-Sepharose chromatography. Sensitivity to these effectors was restored by incubation of the adenylate cyclase preparation with detergent-solubilized protein from bovine cerebral cortex. Reconstitution of of Ca2+.calmodulin activation required the presence of 5'-guanylyl imidodiphosphate. The factor required for restoration of Ca2+.calmodulin stimulation was sensitive to heat, trypsin digestion, and N-ethylmaleimide. These observations suggest that this adenylate cyclase activity requires the presence of one or more guanyl nucleotide binding subunits for calmodulin sensitivity.

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References

    1. Proc Natl Acad Sci U S A. 1979 Jan;76(1):204-8 - PubMed
    1. J Biol Chem. 1979 May 10;254(9):3333-40 - PubMed
    1. J Biol Chem. 1979 Apr 10;254(7):2287-95 - PubMed
    1. Nature. 1978 Apr 20;272(5655):720-2 - PubMed
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