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Case Reports
. 2018 Jan 11:13:2.
doi: 10.1186/s40793-017-0286-7. eCollection 2018.

Draft genome sequence of Streptomyces hyaluromycini MB-PO13T, a hyaluromycin producer

Enjuro Harunari  1 , Hisayuki Komaki  2 , Natsuko Ichikawa  3 , Akira Hosoyama  3 , Akane Kimura  3 , Moriyuki Hamada  2 , Yasuhiro Igarashi  1
Affiliations
Case Reports

Draft genome sequence of Streptomyces hyaluromycini MB-PO13T, a hyaluromycin producer

Enjuro Harunari et al. Stand Genomic Sci. .

Abstract

Streptomyces hyaluromycini MB-PO13T (=NBRC 110483T = DSM 100105T) is type strain of the species, which produces a hyaluronidase inhibitor, hyaluromycin. Here, we report the draft genome sequence of this strain together with features of the organism and generation, annotation and analysis of the genome sequence. The 11.5 Mb genome of Streptomyces hyaluromycini MB-PO13T encoded 10,098 putative ORFs, of which 5317 were assigned with COG categories. The genome harbored at least six type I PKS clusters, three type II PKS gene clusters, two type III PKS gene clusters, six NRPS gene clusters, and one hybrid PKS/NRPS gene cluster. The type II PKS gene cluster including 2-amino-3-hydroxycyclopent-2-enone synthetic genes was identified to be responsible for hyaluromycin synthesis. We propose the biosynthetic pathway based on bioinformatic analysis.

Keywords: Biosynthesis; C5N; Polyketide synthase; Rubromycin; Streptomyces.

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Conflict of interest statement

The authors declare no competing interest.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Scanning electron micrograph of Streptomyces hyaluromycini MB-PO13T grown on 1/10 ISP 2 agar for 14 days at 28 °C. Bar, 5 μm
Fig. 2
Fig. 2
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences of strain MB-PO13T and its taxonomic neighbors. Kitasatospora setae KM-6054T (AB022868) was used as the outgroup. Bootstrap values (>70%) based on 1000 replicates are shown at branch nodes. Bar, 0.01 substitutions per nucleotide position
Fig. 3
Fig. 3
Gene organizations of rubromycin-, hyarulomycin- and griseorhodin-biosynthetic gene clusters. Homologs are linked by gray dotted lines. The rub, Orf1- and grh are rubromycin-, hyarulomycin- and griseorhodin-biosynthetic gene clusters, respectively. Hyarulomycin-biosynthetic genes are indicated with orf numbers as shown in Table 5
Fig. 4
Fig. 4
Putative biosynthetic pathways of hyarulomycin, rubromycin and griseorhodin. Each step is catalyzed by enzymes encoded following genes as proposed in griseorhodin biosynthesis [4]. 1grhA/rubA/orf1-742 (KSα), grhB/rubB/orf1-741 (KSβ) and grhC/rubC/orf1-740 (ACP); 2grhE/rubK?/orf1-769?, grhQ/rubE/orf1-744, grhS/rubD/orf1-743 and grhT/rubF/orf1-739, 3grhO8/rubO/orf1-750, grhO9/rubP/orf1-749 (monooxygenases), grhL/−/orf1-751 (MT), grhM/rubQ/orf1-748 (unknown) and grhP/rubR/orf1-746 (asparagine synthase); 4grhO5/rubL/orf1-755 (monooxygenase) and grhO1/rubI/orf1-758 (oxygenase)?; 5grhO6/rubN/orf1-753 (monooxygenase) and grhJ/−/orf1-756 (acetyltransferase)?; 6grhO10/rubG/orf1-738 (KR) or grhT/rubF/orf1-739 (cyclase/reductase); 7grhO3/rubU/orf1-735 (cytochrome P450), grhO4/−/− (ferredoxin) and grhO7/−/orf1-752 (oxidoreductase). Homologs are connected with slashes in order of rubromycin/griseorhodin/hyarulomycin. ACP, acyl carrier protein; CLF, chain length factor; Fd, ferredoxin; KS, ketosynthase; KR, ketoreductase; MT, methyltransferase; −, no homolog in the sequence
See this image and copyright information in PMC

References

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