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Review
. 2018 Jan 17;7(1):1422676.
doi: 10.1080/20013078.2017.1422676. eCollection 2018.

Therapeutic biomaterials based on extracellular vesicles: classification of bio-engineering and mimetic preparation routes

Pablo García-Manrique  1   2 , María Matos  2 , Gemma Gutiérrez  2 , Carmen Pazos  2 , María Carmen Blanco-López  1
Affiliations
Review

Therapeutic biomaterials based on extracellular vesicles: classification of bio-engineering and mimetic preparation routes

Pablo García-Manrique et al. J Extracell Vesicles. .

Abstract

Extracellular vesicles (EVs) are emerging as novel theranostic tools. Limitations related to clinical uses are leading to a new research area on design and manufacture of artificial EVs. Several strategies have been reported in order to produce artificial EVs, but there has not yet been a clear criterion by which to differentiate these novel biomaterials. In this paper, we suggest for the first time a systematic classification of the terms used to build up the artificial EV landscape, based on the preparation method. This could be useful to guide the derivation to clinical trial routes and to clarify the literature. According to our classification, we have reviewed the main strategies reported to date for their preparation, including key points such as: cargo loading, surface targeting strategies, purification steps, generation of membrane fragments for the construction of biomimetic materials, preparation of synthetic membranes inspired in EV composition and subsequent surface decoration.

Keywords: artificial extracellular vesicles; biomimetic materials; drug-delivery nanocarrier; nanomedicine.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Artificial EV landscape: explored routes to date for the preparation of artificial EVs for specific purposes. *EBSSNs [19].
See this image and copyright information in PMC

References

    1. Yáñez-Mó M, Siljander PRM, Andreu Z, et al. Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles. 2015;4:27066. - PMC - PubMed
    1. Lener T, Gimona M, Aigner L, et al. Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper. J Extracell Vesicles. 2015;4:30087. - PMC - PubMed
    1. Tran TH, Mattheolabakis G, Aldawsari H, et al. Exosomes as nanocarriers for immunotherapy of cancer and inflammatory diseases. Clin Immunol. 2015;160:46–18. - PubMed
    1. Aline F, Bout D, Amigorena S, et al. Toxoplasma gondii antigen-pulsed-dendritic cell-derived exosomes induce a protective immune response against T. gondii infection. Infect Immun. 2004;74:4127–4137. - PMC - PubMed
    1. Dalal J, Gandy K, Domen J.. Role of mesenchymal stem cell therapy in Crohn’s disease. Pediatr Res. 2012;71:445–451. - PubMed