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. 2018 Jan 26;18(2):357.
doi: 10.3390/s18020357.

Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

Shuai Liu  1 , Zhaoyang Tong  2 , Xihui Mu  3 , Bing Liu  4 , Bin Du  5 , Zhiwei Liu  6 , Chuan Gao  7
Affiliations

Detection of Abrin by Electrochemiluminescence Biosensor Based on Screen Printed Electrode

Shuai Liu et al. Sensors (Basel). .

Abstract

For the convenience of fast measurement in the outdoor environment, a portable electrochemiluminescence biosensor with the screen-printed electrode as the reaction center was developed, which possesses the characteristics of high sensitivity, small scale, simplified operation and so on, and has been used for in situ detection of abrin. First, combining with magnetic separation technique, the "biotin-avidin" method was used to immobilize the polyclonal antibody (pcAb) on the magnetic microspheres surface as the capture probe. Secondly, the Ru(bpy)₃2+-labeled monoclonal antibody (mcAb) was used as the specific electrochemiluminescence signal probe. Then, the "mcAb-toxin-pcAb" sandwich model was built to actualize the quantitative detection of abrin on the surface of the screen-printed electrode. The linear detection range was 0.5-1000 ng/mL; the regression equation was Y = 89.251lgX + 104.978 (R = 0.9989, n = 7, p < 0.0001); and the limit of detection (LOD) was 0.1 ng/mL. The sensing system showed high sensitivity, excellent specificity and good anti-interference ability, and could be used for the analysis of trace abrin in various environmental samples with good recovery and reproducibility. Compared with the traditional electrochemiluminescence sensing device, its miniaturization and portability gives it potential to satisfy the requirement of in situ detection.

Keywords: abrin; electrochemiluminescence; portable sensor; screen printed electrode.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Size and real object of screen printed gold electrode (the right is the Chinese currency worth 0.1 Yuan, which is the minimum coin of China in circulation).
Figure 2
Figure 2
Overall structure of portable ECL detection platform: (A) the overall schematic diagram and main modules of the sensor including SPE, PMT, control mainboard, drive and power supply circuit; (B) the main mechanical structure of sensor including hatch door, lead rail, PMT and slider, and the whole shape is a small cube; and(C) a slot for clamping the SPE, which can pass in and out along the lead rail with the assistance of the spring.
Figure 3
Figure 3
Flow diagram of detection of abrin.
Figure 4
Figure 4
The effect of different factors on ECL intensity: (A) the effect of additive amount of sample on ECL intensity; (B) the effect of sample’s pH on ECL intensity; (C) the ECL intensity (C-a) and noise (C-b) of sample in different multiplier series of PMT.
Figure 5
Figure 5
Absorbance spectrum of pcAb solution: before (a); and after (b) binding with magnet microspheres.
Figure 6
Figure 6
Absorbance of mcAb before (b) and after (c) labeled by Ru(bpy)32+-NHS (a).
Figure 7
Figure 7
The real-time ECL scanning image of the labeled probe.
Figure 8
Figure 8
The ECL spectra for the abrin detection at different concentrations.
Figure 9
Figure 9
The relationship between ECL intensity and the concentration of abrin.
See this image and copyright information in PMC

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