Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Mar;15(3):173-182.
doi: 10.1038/nmeth.4593. Epub 2018 Jan 29.

STED super-resolved microscopy

Giuseppe Vicidomini  1 , Paolo Bianchini  2 , Alberto Diaspro  2   3
Affiliations

STED super-resolved microscopy

Giuseppe Vicidomini et al. Nat Methods. 2018 Mar.

Abstract

Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity. However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in the past. Here we summarize the progress that is mitigating these problems and giving substantial momentum to STED microscopy applications. We discuss the future of this method in regard to spatiotemporal limits, live-cell imaging and combination with spectroscopy. Advances in these areas may elevate STED microscopy to a standard method for imaging in the life sciences.

PubMed Disclaimer

References

    1. Biophys J. 2011 Sep 7;101(5):1277-84 - PubMed
    1. Nat Methods. 2015 Sep;12(9):827-30 - PubMed
    1. J Biol Chem. 2016 Aug 12;291(33):16948-62 - PubMed
    1. Nat Commun. 2015 Aug 13;6:7977 - PubMed
    1. Nature. 2017 Mar 9;543(7644):229-233 - PubMed

MeSH terms

LinkOut - more resources