Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan;6(1):8-15.
doi: 10.29252/beat-060102.

Effects of Aloe Vera and Chitosan Nanoparticle Thin-Film Membranes on Wound Healing in Full Thickness Infected Wounds with Methicillin Resistant Staphylococcus Aureus

Reza Ranjbar  1 , Alireza Yousefi  2
Affiliations

Effects of Aloe Vera and Chitosan Nanoparticle Thin-Film Membranes on Wound Healing in Full Thickness Infected Wounds with Methicillin Resistant Staphylococcus Aureus

Reza Ranjbar et al. Bull Emerg Trauma. 2018 Jan.

Abstract

Objective: To assess effect of Aleo vera with chitosan nanoparticle biofilm on wound healing in full thickness infected wounds with antibiotic resistant gram positive bacteria.

Method: Thirty rats were randomized into five groups of six rats each. Group I: Animals with uninfected wounds treated with 0.9% saline solution. Group II: Animals with infected wounds treated with saline. Group III: Animals with infected wounds were dressed with chitosan nanoparticle thin-film membranes. Group IV: Animals with infected wounds were treated topically with Aloe vera and Group V: Animals with infected wounds were treated topically with Aloe vera and dressed with chitosan nanoparticle thin-film membranes. Wound size was measured on 6, 9, 12, 15, 18 and 21days after surgery.

Results: Microbiology, reduction in wound area and hydroxyproline contents indicated that there was significant difference (p<0.05) between group V and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (p<0.05) between group V and other groups.

Conclusion: The Aloe vera with chitosan nanoparticle thin-film membranes had a reproducible wound healing potential and hereby justified its use in practice.

Keywords: Aloe vera; Chitosan nanoparticle; MRSA; Rat; Thin-film membrane; Wound.

PubMed Disclaimer

Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
Box-and-whisker plots of number of polymorphnuclear cells (PMN) in excisional model of the rat’s skin in experimental groups. Results were expressed as mean ± SEM
Fig. 2
Fig. 2
Line graph indicating number of mononuclear cells (MNC) in excisional model of the rat’s skin in experimental groups. Results were expressed as mean ± SEM. *P < 0.05 vs other experimental groups
Fig. 3
Fig. 3
Box-and-whisker plots of number of fibroblasts in excisional model of the rat’s skin in experimental groups. Results were expressed as mean ± SEM
Fig. 4
Fig. 4
Histological characteristics of rat skin on the 7th (A-C) and 14th day (D-F) after wound creation in excisional wound model. A and D: III, B and E: IV, C and F: V. Wounds with surrounding skin were prepared for histological microscopic evaluation by Masson trichrome staining. (×400
See this image and copyright information in PMC

References

    1. Cotran R, Kumar V, Collins T, Robbins SL. Pathologic basis of disease. 6th ed. Philadelphia: Saunders; 1999.
    1. Rajan S. Skin and soft-tissue infections: classifying and treating a spectrum. Cleve Clin J Med. 2012;79(1):57–66. - PubMed
    1. Zetola N, Francis JS, Nuermberger EL, Bishai WR. Community-acquired meticillin-resistant Staphylococcus aureus: an emerging threat. Lancet Infect Dis. 2005;5(5):275–86. - PubMed
    1. Ranjbar R, Takhtfooladi MA. The effects of low level laser therapy on Staphylococcus aureus infected third-degree burns in diabetic rats. Acta Cir Bras. 2016;31(4):250–5. - PubMed
    1. Ranjbar R, Takhtfooladi MA. The effects of photobiomodulation therapy on Staphylococcus aureus infected surgical wounds in diabetic rats A microbiological, histopathological, and biomechanical study. Acta Cir Bras. 2016;31(8):498–504. - PubMed

LinkOut - more resources