Programmed cell death in wheat (Triticum aestivum L.) endosperm cells is affected by drought stress

Abstract

Drought frequently occurs during wheat (Triticum aestivum L.) grain filling. The objectives of this study were (i) to investigate the effect of post-anthesis drought on programmed cell death (PCD) in wheat endosperm cells and (ii) to examine the role of ethylene (ETH) receptors and abscisic acid (ABA) in regulating wheat endosperm PCD. Two winter wheat cultivars ('Xindong 18' and 'Xindong 22') were used in this study. Grain samples were collected from normal and drought stressed plants at 5-day intervals between 5 and 35 days post-anthesis. The samples were then compared with respect to cell viability, nuclear morphometry, cell ultrastructure, DNA integrity, nucleic acid content, and nuclease activity. Analysis was also conducted about gene transcripts related to PCD, ETH receptors, and ABA biosynthesis and degradation. Drought stress reduced cell viability, accelerated nuclear deformation, and increased mitochondrial dissolution. The activity of nucleic acid hydrolase was greater, and the nucleic acid concentrations were less in the drought treatments than in the control. As a result, the peak in DNA fragmentation occurred earlier in the drought treatment. Drought stress significantly increased the expression of four genes related to ABA (nced1, nced2, ao1, ao2). In contrast, drought significantly reduced the expression of four genes related to ETH receptors (ers1, ers2 etr1, etr2) and one gene related to PCD (dad1). In summary, the results indicated that drought stress caused PCD to occur earlier in the endosperm of winter wheat.

Keywords: Drought; Endosperm cell; Ethylene receptor; Programmed cell death; Triticum aestivum L.