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. 1986 Apr;83(7):2037-41.
doi: 10.1073/pnas.83.7.2037.

Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking

P Chaussepied, D Mornet, R Kassab

Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking

P Chaussepied et al. Proc Natl Acad Sci U S A. 1986 Apr.

Abstract

When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N'-p-phenylenedimaleimide or 5,5'-dithiobis(2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a significant amount of Mg2+-nucleotide in the ATPase site.

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