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Comparative Study
. 2018 Jan;82(1):55-59.

Comparison of the oral and rectal mucosal and colonic serosal microcirculations of healthy, anesthetized horses

Philip J Kieffer  1 , Jarred M Williams  1 , Molly K Shepard  1 , Steeve Giguère  1 , Kira L Epstein  1
Affiliations
Comparative Study

Comparison of the oral and rectal mucosal and colonic serosal microcirculations of healthy, anesthetized horses

Philip J Kieffer et al. Can J Vet Res. 2018 Jan.

Abstract
in English, French

The objectives of the study were to: i) determine baseline microvascular perfusion indices (MPI) and assess their repeatability in healthy horses under general anesthesia, and ii) compare the MPIs of 3 microvascular beds (oral mucosa, colonic serosa, and rectal mucosa). Healthy adult horses were anesthetized and sidestream dark field microscopy was used to collect video loops of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions without cardiovascular support drugs; videos were later analyzed to produce MPIs. Baseline MPI values were determined for each site, which included the total vessel density (TVD), perfused vessel density (PVD), portion perfused vessels (PPV), and microcirculatory flow index (MFI). Differences in MPIs between microvascular beds were not statistically significant. Repeatability of the measurements varied for each MPI. In particular, the site of sampling had a profound effect on the repeatability of the PPV measurements and should be considered in future studies.

Les objectifs de cet étude étaient: i) de déterminer les indices de perfusion microvasculaires (IPM) de base et évaluer leur répétabilité chez les chevaux en bonne santé sous anesthésie générale, et ii) de comparer les IPMs de trois lits microvasculaires (muqueuse orale, séreuse du colon, et muqueuse rectale). Des chevaux adultes en bonne santé ont été anesthésiés et une unité de microscopie au champ sombre a été utilisée pour recueillir des boucles vidéo de la muqueuse buccale, de la muqueuse rectale, et de la séreuse du colon sous des conditions de tension artérielle normale. Les vidéos ont été analysées pour produire les IPMs, incluant la densité totale des vaisseaux, la densité des vaisseaux perfusés, la portion des vaisseaux perfusés, et l’index de flux microcirculaire. Pour chaque IPM, les différences entre les sites anatomiques n’étaient pas significatives statistiquement. La répétabilité des mesures variait pour chaque IPM. En particulier, le type de lit microvasculaire a une influence profonde sur la répétabilité des mesures.(Traduit par les auteurs).

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Figures

Figure 1
Figure 1
A — Total Vessel Density (TVD) (mm/mm2). Comparison of the mean and standard deviation (error bars) of the total vessel density of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions. B — Perfused Vessel Density (PVD) (mm/mm2). Comparison of the mean and standard deviation (error bars) of the perfused vessel density of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions. C — Proportion Perfused Vessels (PPV) (%). Comparison of the mean and standard deviation (error bars) of the proportion of perfused vessels of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions. D — Microvascular Flow Indices (MFI). A comparison of the mean and standard deviation (error bars) of the microvascular flow index of the oral mucosa, rectal mucosa, and colonic serosa under normotensive conditions.
Figure 2
Figure 2
Coefficient of variations for the microvascular perfusion indices (MPIs). Comparison of the mean coefficients of variation for the MPIs at each site. Error bars represent standard deviation from the mean.
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