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. 1986 Feb 1;152(2):211-4.
doi: 10.1016/0003-2697(86)90399-4.

Sequential detection of antigens in Western blots with differently colored products

N Theisen, E S Lohoff, K von Figura, A Hasilik

Sequential detection of antigens in Western blots with differently colored products

N Theisen et al. Anal Biochem. .

Abstract

A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.

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