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. 2017 Sep-Oct;20(5):346-350.
doi: 10.4103/JCD.JCD_253_16.

Evaluation of new technique of sterilization using biological indicator

Nomal Chintan Sheth  1 , Yogesh V Rathod  1 , Pratima R Shenoi  1 , Deepa D Shori  1 , Rajiv T Khode  1 , Amruta P Khadse  1
Affiliations

Evaluation of new technique of sterilization using biological indicator

Nomal Chintan Sheth et al. J Conserv Dent. 2017 Sep-Oct.

Abstract

Background: A novel technique of sterilization of endodontic files is introduced in this article.

Aims: Newly introduced sterilization unit, named "SteriFast" is compared with autoclave and glass bead sterilizer using biological indicator.

Materials and methods: Spore strips of Bacillus pumilus were cultured in nutrient broth. This cultured media was used to contaminate the experimental samples of endodontic files. These contaminated files were sterilized using three different techniques. The sterilized files were transferred into nutrient medium under aseptic condition. The results were observed after 24 h, 48 h, and 7 days.

Results: The results showed that autoclave and new sterilization device (SteriFast) showed complete sterilization. The files sterilized using glass bead sterilizer showed bacterial growth (80%).

Conclusions: Thus, it proves that autoclave and SteriFast are ideal techniques of sterilization of endodontic files. Glass bead sterilizer does not completely sterilize the files. The article also compares SteriFast and autoclave in other aspects such as its design, basic principle, advantages, and disadvantages. The article also describes features and design of SteriFast, used for all kind of small dental instruments.

Keywords: Autoclave; Bacillus pumilus; SteriFast; biological indicator; sterilization; ultraviolet germicidal irradiation.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Division of groups
Figure 2
Figure 2
Results. (a) Sample showing turbidity has bacterial growth (b) Sample with no turbidity has no bacterial growth, hence proved 100% sterile (c) Schematic representation of the results in each group
See this image and copyright information in PMC

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