Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:8949264.
doi: 10.1155/2017/8949264. Epub 2017 Dec 13.

Cell Colonization Ability of a Commercialized Large Porous Alveolar Scaffold

S Lemonnier  1 , T Bouderlique  2   3   4 , S Naili  1 , H Rouard  3 , J Courty  2 , N Chevallier  3 , P Albanese  2 , T Lemaire  1
Affiliations

Cell Colonization Ability of a Commercialized Large Porous Alveolar Scaffold

S Lemonnier et al. Appl Bionics Biomech. 2017.

Abstract

The use of filling biomaterials or tissue-engineered large bone implant-coupling biocompatible materials and human bone marrow mesenchymal stromal cells seems to be a promising approach to treat critical-sized bone defects. However, the cellular seeding onto and into large porous scaffolds still remains challenging since this process highly depends on the porous microstructure. Indeed, the cells may mainly colonize the periphery of the scaffold, leaving its volume almost free of cells. In this study, we carry out an in vitro study to analyze the ability of a commercialized scaffold to be in vivo colonized by cells. We investigate the influence of various physical parameters on the seeding efficiency of a perfusion seeding protocol using large manufactured bone substitutes. The present study shows that the velocity of the perfusion fluid and the initial cell density seem to impact the seeding results and to have a negative effect on the cellular viability, whereas the duration of the fluid perfusion and the nature of the flow (steady versus pulsed) did not show any influence on either the fraction of seeded cells or the cellular viability rate. However, the cellular repartition after seeding remains highly heterogeneous.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Experimental setup: A, general view of the perfusion chamber connected to the peristaltic pump; B, zoom in on the scaffold inside the flow chamber; C, view of the scaffold inside its silicon protective duct.
Figure 2
Figure 2
Influence of the initial cell number Ncell and the nature of the flow on the seeding efficiency.
Figure 3
Figure 3
Influence of the sedimentation time Tp on the seeding results.
Figure 4
Figure 4
Influence of the perfusion distance dfl on the seeding results.
Figure 5
Figure 5
Influence of the fluid velocity Vfl on the seeding results. Tests with Vfl = 3.17 × 10−4 m/s and Vfl = 9.7 × 10−4 m/s have been conducted 3 times, whereas the static test and the test with Vfl = 1.73 × 10−4 m/s have only been conducted once as comparison tools.
Figure 6
Figure 6
Histological slice of a scaffold seeded under controlled fluid perfusion.
Figure 7
Figure 7
Different types of scaffold macroscopic structures.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Schroeder J. E., Mosheiff R. Tissue engineering approaches for bone repair: concepts and evidence. Injury. 2011;42(6):609–613. doi: 10.1016/j.injury.2011.03.029. - DOI - PubMed
    1. Panagiotis M. Classification of non-union. Injury. 2005;36(4):S30–S37. doi: 10.1016/j.injury.2005.10.008. - DOI - PubMed
    1. Sen M. K., Miclau T. Autologous iliac crest bone graft: should it still be the gold standard for treating nonunions? Injury. 2007;38(1):S75–S80. doi: 10.1016/j.injury.2007.02.012. - DOI - PubMed
    1. Soucacos P. N., Dailiana Z., Beris A. E., Johnson E. O. Vascularised bone grafts for the management of non-union. Injury. 2006;37(1):S41–S50. doi: 10.1016/j.injury.2006.02.040. - DOI - PubMed
    1. Petite H., Viateau V., Bensaïd W., et al. Tissue-engineered bone regeneration. Nature Biotechnology. 2000;18(9):959–963. doi: 10.1038/79449. - DOI - PubMed