Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review

Abstract

Nuclear transfer procedures have been recently applied for clinical and research targets as a novel assisted reproductive technique and were used for increasing the oocyte activity during its growth and maturation. In this review, we summarized the nuclear transfer technique for germinal vesicle stage oocytes to reconstruct the maturation of them. Our study covered publications between 1966 and August 2017. In result utilized germinal vesicle transfer techniques, fusion, and fertilization survival rate on five different mammalian species are discussed, regarding their potential clinical application. It seems that with a study on this method, there is real hope for effective treatments of old oocytes or oocytes containing mitochondrial problems in the near future.

Keywords: Germinal vesicle; Micromanipulation; Nuclear transfer; Oocyte.

Figure 1

Literature search; the search covered the years 1966 to August 2017. A total of 18,901 articles reviewed, 137 articles were selected. Among these 137 selected articles which one was irrelevant, duplicate or publication with duplicate data was excluded, so 52 articles remained.