doi: 10.1007/978-1-4939-7652-2_6.
Isolation of Extracellular RNA from Bile
Affiliations
- PMID: 29388136
- DOI: 10.1007/978-1-4939-7652-2_6
Item in Clipboard
Isolation of Extracellular RNA from Bile
Methods Mol Biol.
2018.
Abstract
The study of extracellular RNA has been recently reported as a tool for biomarker discovery. Extracellular vesicles can be isolated from different types of body fluids which contain protein, mRNA, and noncoding RNA. Extracellular RNA isolated from bile could be a useful tool for analyzing biliary tract diseases or cancer. Herein, we describe protocols based on modifications of commercially available kits for the collection, processing, and isolation of extracellular RNA from bile.
Keywords: Bile; Exosomes; Extracellular RNA; Extracellular Vesicles; Isolation.
Similar articles
-
Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles.Methods Mol Biol. 2018;1740:17-21. doi: 10.1007/978-1-4939-7652-2_2. Methods Mol Biol. 2018. PMID: 29388132 Free PMC article. Review.
-
Extracellular RNA Isolation from Cell Culture Supernatant.Methods Mol Biol. 2018;1740:23-34. doi: 10.1007/978-1-4939-7652-2_3. Methods Mol Biol. 2018. PMID: 29388133
-
Isolation of Extracellular RNA from Serum/Plasma.Methods Mol Biol. 2018;1740:43-57. doi: 10.1007/978-1-4939-7652-2_5. Methods Mol Biol. 2018. PMID: 29388135 Free PMC article.
-
Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis.Methods Mol Biol. 2017;1660:55-63. doi: 10.1007/978-1-4939-7253-1_6. Methods Mol Biol. 2017. PMID: 28828648
-
Extracellular RNAs: A New Awareness of Old Perspectives.Methods Mol Biol. 2018;1740:1-15. doi: 10.1007/978-1-4939-7652-2_1. Methods Mol Biol. 2018. PMID: 29388131 Free PMC article. Review.
Cited by
-
Liquid biopsy in cholangiocarcinoma: Current status and future perspectives.World J Gastrointest Oncol. 2021 May 15;13(5):332-350. doi: 10.4251/wjgo.v13.i5.332. World J Gastrointest Oncol. 2021. PMID: 34040697 Free PMC article. Review.
-
Roles of Bile-Derived Exosomes in Hepatobiliary Disease.Biomed Res Int. 2021 Jan 13;2021:8743409. doi: 10.1155/2021/8743409. eCollection 2021. Biomed Res Int. 2021. PMID: 33511212 Free PMC article. Review.
-
The Diagnostic and Prognostic Value of Plasmatic Exosome Count in Cancer Patients and in Patients with Other Pathologies.Int J Mol Sci. 2024 Jan 15;25(2):1049. doi: 10.3390/ijms25021049. Int J Mol Sci. 2024. PMID: 38256122 Free PMC article.
-
The Importance of Detecting, Quantifying, and Characterizing Exosomes as a New Diagnostic/Prognostic Approach for Tumor Patients.Cancers (Basel). 2023 May 23;15(11):2878. doi: 10.3390/cancers15112878. Cancers (Basel). 2023. PMID: 37296842 Free PMC article. Review.
-
Validation of RNA Extraction Methods and Suitable Reference Genes for Gene Expression Studies in Developing Fetal Human Inner Ear Tissue.Int J Mol Sci. 2024 Mar 2;25(5):2907. doi: 10.3390/ijms25052907. Int J Mol Sci. 2024. PMID: 38474154 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
